Human GNMT ELISA Kit

Alternative name
Human HEL-S-182mP ELISA Kit;Human glycine N-methyltransferase ELISA Kit;Human epididymis secretory sperm binding protein Li 182mP ELISA Kit;
Catalog
E017033
species
Human
GeneGNMT
SpecificityThis assay has high sensitivity and excellent specificity for detection of GNMT. No significant cross-reactivity or interference between GNMT and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human GNMT is typically less than 0.101ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addi
Intended UseHuman GNMT ELISA Kit allows for the in vitro quantitative determination of GNMT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GNMT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GNMT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GNMT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GNMT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Glycine N-methyltransferase Protein information
Uniprot ID GNMT_HUMAN
Uniprot AC Q14749; Q5T8W2; Q9NNZ1; Q9NS24;
UniGene Hs.144914;
GeneID 27232
KEGG hsa:27232;
Human Glycine N-methyltransferase Protein SEQUENCE
SEQUENCE 295 AA; 32742 MW; 34F4546136FD27ED CRC64;
MVDSVYRTRS LGVAAEGLPD QYADGEAARV WQLYIGDTRS RTAEYKAWLL
GLLRQHGCQR VLDVACGTGV DSIMLVEEGF SVTSVDASDK MLKYALKERW
NRRHEPAFDK WVIEEANWMT LDKDVPQSAE GGFDAVICLG NSFAHLPDCK
GDQSEHRLAL KNIASMVRAG GLLVIDHRNY DHILSTGCAP PGKNIYYKSD
LTKDVTTSVL IVNNKAHMVT LDYTVQVPGA GQDGSPGLSK FRLSYYPHCL
ASFTELLQAA FGGKCQHSVL GDFKPYKPGQ TYIPCYFIHV LKRTD

Category

Human GNMT ELISA Kit

Human HEL-S-182mP ELISA Kit;Human glycine N-methyltransferase ELISA Kit;Human epididymis secretory sperm binding protein Li 182mP ELISA Kit;