Human IL6ST ELISA Kit

Alternative name
Human IL-6 receptor subunit beta ELISA Kit;Human IL-6R subunit beta ELISA Kit;Human IL-6R-beta ELISA Kit;Human IL-6RB ELISA Kit;Human CDW130 ELISA Kit;Human Interleukin-6 signal transducer ELISA Kit;Human membrane glycoprotein 130 ELISA Kit;Human GP130 ELISA Kit;Human oncostatin-M receptor subunit alpha ELISA Kit;Human CD130 ELISA Kit;Human interleukin 6 signal transducer ELISA Kit;Human interleukin-6 receptor subunit beta ELISA Kit;Human CD130 antigen ELISA Kit;Human gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form ELISA Kit;Human gp130, oncostatin M receptor ELISA Kit;Human interleukin receptor beta chain ELISA Kit;Human membrane glycoprotein gp130 ELISA Kit;
Catalog
E017343
species
Human
GeneIL6ST
SpecificityNatural and recombinant human sGP130
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman IL6ST ELISA Kit allows for the in vitro quantitative determination of IL6ST , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human GP130 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GP130 has been precoated onto 96-well plates. Standards(sf21,L24-E619) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GP130 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human GP130 amount of sample captured in plate. Background: GP130(Glycoprotein 130) also known as IL6ST or CD130, is a transmembrane protein which is the founding member of the class of all cytokine receptors. The GP130 gene is mapped on 5q11.2. Gp130 showed an apparent molecular mass of 130 kD by SDS-PAGE. Gp130 is involved in the formation of high-affinity IL6-binding sites and in IL6 signal transduction. Sgp130 has a role in modulating signals transduced by membrane-bound gp130. Gp130 is an important part of many different types of signaling complexes.Gp130 molecules with mutations in either of these segments could not transduce a growth signal.
Human Interleukin-6 receptor subunit beta Protein information
Uniprot ID IL6RB_HUMAN
Uniprot AC P40189; A0N0L4; Q5FC04; Q9UQ41;
UniGene Hs.532082; Hs.706627;
GeneID 3572
KEGG hsa:3572;
Human Interleukin-6 receptor subunit beta Protein SEQUENCE
SEQUENCE 918 AA; 103537 MW; 6510A4409FFCF08C CRC64;
MLTLQTWLVQ ALFIFLTTES TGELLDPCGY ISPESPVVQL HSNFTAVCVL
KEKCMDYFHV NANYIVWKTN HFTIPKEQYT IINRTASSVT FTDIASLNIQ
LTCNILTFGQ LEQNVYGITI ISGLPPEKPK NLSCIVNEGK KMRCEWDGGR
ETHLETNFTL KSEWATHKFA DCKAKRDTPT SCTVDYSTVY FVNIEVWVEA
ENALGKVTSD HINFDPVYKV KPNPPHNLSV INSEELSSIL KLTWTNPSIK
SVIILKYNIQ YRTKDASTWS QIPPEDTAST RSSFTVQDLK PFTEYVFRIR
CMKEDGKGYW SDWSEEASGI TYEDRPSKAP SFWYKIDPSH TQGYRTVQLV
WKTLPPFEAN GKILDYEVTL TRWKSHLQNY TVNATKLTVN LTNDRYLATL
TVRNLVGKSD AAVLTIPACD FQATHPVMDL KAFPKDNMLW VEWTTPRESV
KKYILEWCVL SDKAPCITDW QQEDGTVHRT YLRGNLAESK CYLITVTPVY
ADGPGSPESI KAYLKQAPPS KGPTVRTKKV GKNEAVLEWD QLPVDVQNGF
IRNYTIFYRT IIGNETAVNV DSSHTEYTLS SLTSDTLYMV RMAAYTDEGG
KDGPEFTFTT PKFAQGEIEA IVVPVCLAFL LTTLLGVLFC FNKRDLIKKH
IWPNVPDPSK SHIAQWSPHT PPRHNFNSKD QMYSDGNFTD VSVVEIEAND
KKPFPEDLKS LDLFKKEKIN TEGHSSGIGG SSCMSSSRPS ISSSDENESS
QNTSSTVQYS TVVHSGYRHQ VPSVQVFSRS ESTQPLLDSE ERPEDLQLVD
HVDGGDGILP RQQYFKQNCS QHESSPDISH FERSKQVSSV NEEDFVRLKQ
QISDHISQSC GSGQMKMFQE VSAADAFGPG TEGQVERFET VGMEAATDEG
MPKSYLPQTV RQGGYMPQ
UCSC uc003jqq.4; human. [P40189-1];

Category

Human IL6ST ELISA Kit