Human GDF11 ELISA Kit

Alternative name
Human GDF-11 ELISA Kit;Human bone morphogenetic protein 11 ELISA Kit;Human BMP-11 ELISA Kit;Human BMP11 ELISA Kit;Human growth differentiation factor 11 ELISA Kit;Human growth/differentiation factor 11 ELISA Kit;
Catalog
E017524
species
Human
GeneGDF11
SpecificityThis assay has high sensitivity and excellent specificity for detection of GDF-11. No significant cross-reactivity or interference between GDF-11 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GDF-11 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman GDF11 ELISA Kit allows for the in vitro quantitative determination of GDF11 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCancer
Product Description
specificalPrinciple of the assay: GDF-11 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GDF-11. Standards or samples are then added to the microtiter plate wells and GDF-11 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GDF-11 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GDF-11 are added to each well to "sandwich" the GDF-11 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GDF-11 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GDF-11 concentration in each sample is interpolated from this standard curve.
Human Growth/differentiation factor 11 Protein information
Uniprot ID GDF11_HUMAN
Uniprot AC O95390; Q9UID1; Q9UID2;
UniGene Hs.600883;
GeneID 10220
KEGG hsa:10220;
Human Growth/differentiation factor 11 Protein SEQUENCE
SEQUENCE 407 AA; 45091 MW; E8FF48E363635BA8 CRC64;
MVLAAPLLLG FLLLALELRP RGEAAEGPAA AAAAAAAAAA AGVGGERSSR
PAPSVAPEPD GCPVCVWRQH SRELRLESIK SQILSKLRLK EAPNISREVV
KQLLPKAPPL QQILDLHDFQ GDALQPEDFL EEDEYHATTE TVISMAQETD
PAVQTDGSPL CCHFHFSPKV MFTKVLKAQL WVYLRPVPRP ATVYLQILRL
KPLTGEGTAG GGGGGRRHIR IRSLKIELHS RSGHWQSIDF KQVLHSWFRQ
PQSNWGIEIN AFDPSGTDLA VTSLGPGAEG LHPFMELRVL ENTKRSRRNL
GLDCDEHSSE SRCCRYPLTV DFEAFGWDWI IAPKRYKANY CSGQCEYMFM
QKYPHTHLVQ QANPRGSAGP CCTPTKMSPI NMLYFNDKQQ IIYGKIPGMV
VDRCGCS
UCSC uc001shq.4; human.;

Category

Human GDF11 ELISA Kit

Human GDF-11 ELISA Kit;Human bone morphogenetic protein 11 ELISA Kit;Human BMP-11 ELISA Kit;Human BMP11 ELISA Kit;Human growth differentiation factor 11 ELISA Kit;Human growth/differentiation factor 11 ELISA Kit;