androstenedioneprostaglandin e2galectin-3hyaluronic acidasprosin

Home Human GDF9 ELISA Kit

Category

Human GDF9 ELISA Kit

Contact Us
  • Alternative name

    Human GDF-9 ELISA Kit;Human growth differentiation factor 9 ELISA Kit;Human growth/differentiation factor 9 ELISA Kit;
  • Catalog
    E017548
  • species
    Human
  • GeneGDF9
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human GDF9. No significant cross-reactivity or interference between Human GDF9 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity21.4 pg/ml
  • Intended UseHuman GDF9 ELISA Kit allows for the in vitro quantitative determination of GDF9 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Introduction: Growth differentiation factor-9 (GDF-9) is an oocyte-derived growth factor and a member of the transforming growth factor- (TGF-) superfamily that includes TGF-, activin, and bone morphogenetic proteins (BMPs). GDF-9 is indispensable for the development of ovarian follicles from the primary stage, and treatment with GDF-9 enhances the progression of early follicles into small preantral follicles. Similar to other TGF- family ligands, GDF-9 likely initiates signaling mediated by type I and type II receptors with serine/threonine kinase activity, followed by the phosphorylation of intracellular transcription factors named Smads. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GDF-9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for GDF-9 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain GDF-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of GDF-9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Growth/differentiation factor 9 Protein information
  • Uniprot ID GDF9_HUMAN
  • Uniprot AC O60383; Q4VAW5;
  • UniGene Hs.25022; Hs.739230; Hs.742490;
  • GeneID 2661
  • KEGG hsa:2661;
  • Human Growth/differentiation factor 9 Protein SEQUENCE

  • SEQUENCE 454 AA; 51444 MW; 94EA366E90CDBC27 CRC64;

    MARPNKFLLW FCCFAWLCFP ISLGSQASGG EAQIAASAEL ESGAMPWSLL

    QHIDERDRAG LLPALFKVLS VGRGGSPRLQ PDSRALHYMK KLYKTYATKE

    GIPKSNRSHL YNTVRLFTPC TRHKQAPGDQ VTGILPSVEL LFNLDRITTV

    EHLLKSVLLY NINNSVSFSS AVKCVCNLMI KEPKSSSRTL GRAPYSFTFN

    SQFEFGKKHK WIQIDVTSLL QPLVASNKRS IHMSINFTCM KDQLEHPSAQ

    NGLFNMTLVS PSLILYLNDT SAQAYHSWYS LHYKRRPSQG PDQERSLSAY

    PVGEEAAEDG RSSHHRHRRG QETVSSELKK PLGPASFNLS EYFRQFLLPQ

    NECELHDFRL SFSQLKWDNW IVAPHRYNPR YCKGDCPRAV GHRYGSPVHT

    MVQNIIYEKL DSSVPRPSCV PAKYSPLSVL TIEPDGSIAY KEYEDMIATK

    CTCR

  • UCSC uc003kxz.3; human.;



Inquiry About Human GDF9 ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!