Human CYP46A1 ELISA Kit

Alternative name
Human CH24H ELISA Kit;Human cytochrome P450 46A1 ELISA Kit;Human CYP46 ELISA Kit;Human CP46 ELISA Kit;Human cytochrome P450 family 46 subfamily A member 1 ELISA Kit;Human cholesterol 24-hydroxylase ELISA Kit;Human cytochrome P450, family 46, subfamily A, polypeptide 1 ELISA Kit;Human cytochrome P450, subfamily 46 (cholesterol 24-hydroxylase) ELISA Kit;
Catalog
E008217
species
Human
GeneCYP46A1
SpecificityThis assay has high sensitivity and excellent specificity for detection of CYP46A1. No significant cross-reactivity or interference between CYP46A1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CYP46A1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CYP46A1 ELISA Kit allows for the in vitro quantitative determination of CYP46A1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: This CYP46A1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CYP46A1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: CYP46A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CYP46A1 antibody and an CYP46A1-HRP conjugate. The assay sample and buffer are incubated together with CYP46A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CYP46A1 concentration since CYP46A1 from samples and CYP46A1-HRP conjugate compete for the anti-CYP46A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CYP46A1 from the sample, fewer sites are left to bind CYP46A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYP46A1 concentration in each sample is interpolated from this standard curve.
Human Cholesterol 24-hydroxylase Protein information
Uniprot ID CP46A_HUMAN
Uniprot AC Q9Y6A2; B4DHP8; E7EQG9; Q8N2B0;
UniGene Hs.25121;
GeneID 10858
KEGG hsa:10858;
Human Cholesterol 24-hydroxylase Protein SEQUENCE
SEQUENCE 500 AA; 56821 MW; AB9307749D9E5FDA CRC64;
MSPGLLLLGS AVLLAFGLCC TFVHRARSRY EHIPGPPRPS FLLGHLPCFW
KKDEVGGRVL QDVFLDWAKK YGPVVRVNVF HKTSVIVTSP ESVKKFLMST
KYNKDSKMYR ALQTVFGERL FGQGLVSECN YERWHKQRRV IDLAFSRSSL
VSLMETFNEK AEQLVEILEA KADGQTPVSM QDMLTYTAMD ILAKAAFGME
TSMLLGAQKP LSQAVKLMLE GITASRNTLA KFLPGKRKQL REVRESIRFL
RQVGRDWVQR RREALKRGEE VPADILTQIL KAEEGAQDDE GLLDNFVTFF
IAGHETSANH LAFTVMELSR QPEIVARLQA EVDEVIGSKR YLDFEDLGRL
QYLSQVLKES LRLYPPAWGT FRLLEEETLI DGVRVPGNTP LLFSTYVMGR
MDTYFEDPLT FNPDRFGPGA PKPRFTYFPF SLGHRSCIGQ QFAQMEVKVV
MAKLLQRLEF RLVPGQRFGL QEQATLKPLD PVLCTLRPRG WQPAPPPPPC
UCSC uc001ygo.4; human. [Q9Y6A2-1];

Category

Human CYP46A1 ELISA Kit