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Human HMGB1 ELISA Kit

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  • Alternative name

    Human High mobility group protein 1 ELISA Kit;Human HMG-1 ELISA Kit;Human HMG1 ELISA Kit;Human HMG3 ELISA Kit;Human SBP-1 ELISA Kit;Human high mobility group box 1 ELISA Kit;Human high mobility group protein B1 ELISA Kit;Human Amphoterin ELISA Kit;Human Sulfoglucuronyl carbohydrate binding protein ELISA Kit;Human high-mobility group (nonhistone chromosomal) protein 1 ELISA Kit;
  • Catalog
    E018616
  • species
    Human
  • GeneHMGB1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of HMGB1. No significant cross-reactivity or interference between HMGB1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HMGB1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman HMGB1 ELISA Kit allows for the in vitro quantitative determination of HMGB1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical    Principle of the assay: HMGB1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HMGB1 antibody and an HMGB1-HRP conjugate. The assay sample and buffer are incubated together with HMGB1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HMGB1 concentration since HMGB1 from samples and HMGB1-HRP conjugate compete for the anti-HMGB1 antibody binding site. Since the number of sites is limited, as more sites are occupied by HMGB1 from the sample, fewer sites are left to bind HMGB1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HMGB1 concentration in each sample is interpolated from this standard curve.
  • Human High mobility group protein B1 Protein information
  • Uniprot ID HMGB1_HUMAN
  • Uniprot AC P09429; A5D8W9; Q14321; Q5T7C3; Q6IBE1;
  • UniGene Hs.434102; Hs.593339; Hs.596078;
  • GeneID 3146
  • KEGG hsa:3146;
  • Human High mobility group protein B1 Protein SEQUENCE

  • SEQUENCE 215 AA; 24894 MW; 8A868CF277D417B5 CRC64;

    MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK

    TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS

    AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK

    LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE

    EDEEDEDEEE DDDDE

  • UCSC uc001usx.5; human.;



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