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Human HNMT ELISA Kit

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  • Alternative name

    Human HMT ELISA Kit;Human HNMT-S1 ELISA Kit;Human HNMT-S2 ELISA Kit;Human MRT51 ELISA Kit;Human histamine N-methyltransferase ELISA Kit;
  • Catalog
    E018685
  • species
    Human
  • GeneHNMT
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human HNMT. No significant cross-reactivity or interference between human HNMT and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human HNMT is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseHuman HNMT ELISA Kit allows for the in vitro quantitative determination of HNMT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HNMT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HNMT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HNMT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HNMT bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Histamine N-methyltransferase Protein information
  • Uniprot ID HNMT_HUMAN
  • Uniprot AC P50135; B2R9J3; Q546Z6; Q7Z7I2; Q8IU56; Q8WW98; Q9BRW6;
  • UniGene Hs.42151;
  • GeneID 3176
  • KEGG hsa:3176;
  • Human Histamine N-methyltransferase Protein SEQUENCE

  • SEQUENCE 292 AA; 33295 MW; 9CCADD1EE0CCB653 CRC64;

    MASSMRSLFS DHGKYVESFR RFLNHSTEHQ CMQEFMDKKL PGIIGRIGDT

    KSEIKILSIG GGAGEIDLQI LSKVQAQYPG VCINNEVVEP SAEQIAKYKE

    LVAKTSNLEN VKFAWHKETS SEYQSRMLEK KELQKWDFIH MIQMLYYVKD

    IPATLKFFHS LLGTNAKMLI IVVSGSSGWD KLWKKYGSRF PQDDLCQYIT

    SDDLTQMLDN LGLKYECYDL LSTMDISDCF IDGNENGDLL WDFLTETCNF

    NATAPPDLRA ELGKDLQEPE FSAKKEGKVL FNNTLSFIVI EA

  • UCSC uc002tvd.4; human. [P50135-1];



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