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Alternative name
Procollagen C-endopeptidase enhancer 1 ELISA KIT; Procollagen COOH-terminal proteinase enhancer 1 ELISA KIT; PCPE-1 ELISA KIT; Procollagen C-proteinase enhancer 1 ELISA KIT; Type 1 procollagen C-proteinase enhancer protein ELISA KIT; Type I procollagen COOH-terminal proteinase enhancerPCOLCE ELISA KIT; PCPE1 ELISA KIT; PCPE-1 ELISA KIT; Procollagen C-proteinase enhancer 1 ELISA KIT
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Catalog
E030891
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species
Human
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GenePCOLCE1
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity40 pg/ml
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Intended UseHuman PCOLCE1 ELISA Kit allows for the in vitro quantitative determination of PCOLCE1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalIntroduction: Procollagen C proteinases (pCPs) cleave type I to III procollagen C propeptides as a necessary step in assembling the major fibrous components of vertebrate extracellular matrix. The protein PCOLCE1 (procollagen C proteinase enhancer 1) is not a proteinase but can enhance the activity of pCPs approximately 10-fold in vitro and has reported roles in inhibiting other proteinases and in growth control.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PCOLCE1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for PCOLCE1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain PCOLCE1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PCOLCE1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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