Human IGFBP4 ELISA Kit

Alternative name
Human IBP-4 ELISA Kit;Human IGF-binding protein 4 ELISA Kit;Human IGFBP-4 ELISA Kit;Human IBP4 ELISA Kit;Human BP-4 ELISA Kit;Human HT29-IGFBP ELISA Kit;Human insulin like growth factor binding protein 4 ELISA Kit;Human insulin-like growth factor-binding protein 4 ELISA Kit;
Catalog
E019294
species
Human
GeneIGFBP4
SpecificityNatural and recombinant human IGFBP4
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity Sample For quantitative detection of human IGFBP4 in cell culture supernates, serum, and pl
Intended UseHuman IGFBP4 ELISA Kit allows for the in vitro quantitative determination of IGFBP4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: Human IGFBP4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP4 has been precoated onto 96-well plates. Standards (NSO, D22-E258) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGFBP4 amount of sample captured in plate. Background: Insulin-like growth factor-binding protein 4 is a protein that in humans is encoded by the IGFBP4gene. This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. In addition, IGFBP-4 is a unique protein and it consistently inhibits several cancer cells in vivo and in vitro. Its inhibitory action has been shown in vivo in prostate and colon. It is secreted by all colon cancer cells.
Human Insulin-like growth factor-binding protein 4 Protein information
Uniprot ID IBP4_HUMAN
Uniprot AC P22692; A0N9W2; B4E351; Q5U012; Q9UCL6;
UniGene Hs.462998;
GeneID 3487
KEGG hsa:3487;
Human Insulin-like growth factor-binding protein 4 Protein SEQUENCE
SEQUENCE 258 AA; 27934 MW; 5E8F4638D99F0A94 CRC64;
MLPLCLVAAL LLAAGPGPSL GDEAIHCPPC SEEKLARCRP PVGCEELVRE
PGCGCCATCA LGLGMPCGVY TPRCGSGLRC YPPRGVEKPL HTLMHGQGVC
MELAEIEAIQ ESLQPSDKDE GDHPNNSFSP CSAHDRRCLQ KHFAKIRDRS
TSGGKMKVNG APREDARPVP QGSCQSELHR ALERLAASQS RTHEDLYIIP
IPNCDRNGNF HPKQCHPALD GQRGKCWCVD RKTGVKLPGG LEPKGELDCH
QLADSFRE
UCSC uc002hus.4; human. [P22692-1];

Category

Human IGFBP4 ELISA Kit

Human IBP-4 ELISA Kit;Human IGF-binding protein 4 ELISA Kit;Human IGFBP-4 ELISA Kit;Human IBP4 ELISA Kit;Human BP-4 ELISA Kit;Human HT29-IGFBP ELISA Kit;Human insulin like growth factor binding protein 4 ELISA Kit;Human insulin-like growth factor-binding protein 4 ELISA Kit;