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Human IL-12 p35 ELISA Kit

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  • Alternative name

    Interleukin-12 subunit alpha ELISA KIT; Cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA KIT; CLMF p35 ELISA KIT; IL-12 subunit p35 ELISA KIT; NK cell stimulatory factor chain 1 ELISA KIT; NKSF1IL12A ELISA KIT; NKSF1 ELISA KIT; IL-12A ELISA KIT; CLMF p35 ELISA KIT; NKSF1 ELISA KIT
  • Catalog
    E019342
  • species
    Human
  • GeneIL-12 p35
  • SpecificityThis kit recognizes natural and recombinant Human IL-12 p35. No significant cross-reactivity or interference between Human IL-12 p35 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of Human IL-12 p35 is 4.688pg/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
  • Intended UseHuman IL-12 p35 ELISA Kit allows for the in vitro quantitative determination of IL-12 p35 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical    Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-12 p35. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-12 p35 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-12 p35, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IL-12 p35. You can calculate the concentration of IL-12 p35 in the samples by comparing the OD of the samples to the standard curve. FOR



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