Human IL34 ELISA Kit

Alternative name
Human IL-34 ELISA Kit;Human C16orf77 ELISA Kit;Human interleukin 34 ELISA Kit;Human interleukin-34 ELISA Kit;
Catalog
E019476
species
Human
GeneIL34
SpecificityThe Human IL-34 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IL-34 proteins within the range of 62.5 pg/ml - 4000 pg/ml.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity10 pg/ml.
Intended UseHuman IL34 ELISA Kit allows for the in vitro quantitative determination of IL34 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||The Human IL-34 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-34 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human IL-34 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-34 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-34 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-34 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Interleukin-34, or IL-34 is a protein belonging to a group of cytokines called interleukins. It is mapped to 16q22.1. The discovery of IL-34 protein in the red pulp of the spleen suggests involvement in growth and development of myeloid cells, consistent with its activity on monocytes. IL-34 is a recently discovered cytokine that acts on tissue resident macrophages and Langerhans cells upon binding the receptor for CSF-1, CSF-1R. It also can promote the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor. In addition, it promotes the development, survival, and function of microglia and Langerhans cells, therefore, this cytokine may predominately function in brain and skin biology.
Human Interleukin-34 Protein information
Uniprot ID IL34_HUMAN
Uniprot AC Q6ZMJ4; B2RC28; B2Z4A8; B2ZC70; Q8N6L2;
UniGene Hs.461214;
GeneID 146433
KEGG hsa:146433;
Human Interleukin-34 Protein SEQUENCE
SEQUENCE 242 AA; 27482 MW; D1D8CC74C77AB23F CRC64;
MPRGFTWLRY LGIFLGVALG NEPLEMWPLT QNEECTVTGF LRDKLQYRSR
LQYMKHYFPI NYKISVPYEG VFRIANVTRL QRAQVSEREL RYLWVLVSLS
ATESVQDVLL EGHPSWKYLQ EVETLLLNVQ QGLTDVEVSP KVESVLSLLN
APGPNLKLVR PKALLDNCFR VMELLYCSCC KQSSVLNWQD CEVPSPQSCS
PEPSLQYAAT QLYPPPPWSP SSPPHSTGSV RPVRAQGEGL LP
UCSC uc002ezh.2; human. [Q6ZMJ4-1];

Category

Human IL34 ELISA Kit

Human IL-34 ELISA Kit;Human C16orf77 ELISA Kit;Human interleukin 34 ELISA Kit;Human interleukin-34 ELISA Kit;