Human CHRM5 ELISA Kit

Alternative name
Human HM5 ELISA Kit;Human cholinergic receptor muscarinic 5 ELISA Kit;Human muscarinic acetylcholine receptor M5 ELISA Kit;Human acetylcholine receptor, muscarinic 5 ELISA Kit;
Catalog
E008272
species
Human
GeneCHRM5
SpecificityThis assay has high sensitivity and excellent specificity for detection of human CHRM5. No significant cross-reactivity or interference between human CHRM5 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human CHRM5 is typically less than 9.75 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseHuman CHRM5 ELISA Kit allows for the in vitro quantitative determination of CHRM5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CHRM5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHRM5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHRM5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHRM5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Muscarinic acetylcholine receptor M5 Protein information
Uniprot ID ACM5_HUMAN
Uniprot AC P08912; Q96RG7;
UniGene Hs.584747;
GeneID 1133
KEGG hsa:1133;
Human Muscarinic acetylcholine receptor M5 Protein SEQUENCE
SEQUENCE 532 AA; 60074 MW; EE846186F645DD9B CRC64;
MEGDSYHNAT TVNGTPVNHQ PLERHRLWEV ITIAAVTAVV SLITIVGNVL
VMISFKVNSQ LKTVNNYYLL SLACADLIIG IFSMNLYTTY ILMGRWALGS
LACDLWLALD YVASNASVMN LLVISFDRYF SITRPLTYRA KRTPKRAGIM
IGLAWLISFI LWAPAILCWQ YLVGKRTVPL DECQIQFLSE PTITFGTAIA
AFYIPVSVMT ILYCRIYRET EKRTKDLADL QGSDSVTKAE KRKPAHRALF
RSCLRCPRPT LAQRERNQAS WSSSRRSTST TGKPSQATGP SANWAKAEQL
TTCSSYPSSE DEDKPATDPV LQVVYKSQGK ESPGEEFSAE ETEETFVKAE
TEKSDYDTPN YLLSPAAAHR PKSQKCVAYK FRLVVKADGN QETNNGCHKV
KIMPCPFPVA KEPSTKGLNP NPSHQMTKRK RVVLVKERKA AQTLSAILLA
FIITWTPYNI MVLVSTFCDK CVPVTLWHLG YWLCYVNSTV NPICYALCNR
TFRKTFKMLL LCRWKKKKVE EKLYWQGNSK LP
UCSC uc001zhk.2; human.;

Category

Human CHRM5 ELISA Kit

Human HM5 ELISA Kit;Human cholinergic receptor muscarinic 5 ELISA Kit;Human muscarinic acetylcholine receptor M5 ELISA Kit;Human acetylcholine receptor, muscarinic 5 ELISA Kit;