Human IDO1 ELISA Kit

Alternative name
Human IDO-1 ELISA Kit;Human indoleamine-pyrrole 2,3-dioxygenase ELISA Kit;Human IDO ELISA Kit;Human INDO ELISA Kit;Human indoleamine 2,3-dioxygenase 1 ELISA Kit;Human indolamine 2,3 dioxygenase ELISA Kit;Human indole 2,3-dioxygenase ELISA Kit;
Catalog
E019703
species
Human
GeneIDO1
SpecificityThis assay has high sensitivity and excellent specificity for detection of IDO1 . No significant cross-reactivity or interference between IDO1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 0.938ng/ml
Intended UseHuman IDO1 ELISA Kit allows for the in vitro quantitative determination of IDO1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IDO1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- IDO1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IDO1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IDO1 can be calculated.
Human Indoleamine 2,3-dioxygenase 1 Protein information
Uniprot ID I23O1_HUMAN
Uniprot AC P14902; E5RGR8; F6M9T7; Q540B4;
UniGene Hs.840;
GeneID 3620
KEGG hsa:3620;
Human Indoleamine 2,3-dioxygenase 1 Protein SEQUENCE
SEQUENCE 403 AA; 45326 MW; E92CF57AD0D0BA8D CRC64;
MAHAMENSWT ISKEYHIDEE VGFALPNPQE NLPDFYNDWM FIAKHLPDLI
ESGQLRERVE KLNMLSIDHL TDHKSQRLAR LVLGCITMAY VWGKGHGDVR
KVLPRNIAVP YCQLSKKLEL PPILVYADCV LANWKKKDPN KPLTYENMDV
LFSFRDGDCS KGFFLVSLLV EIAAASAIKV IPTVFKAMQM QERDTLLKAL
LEIASCLEKA LQVFHQIHDH VNPKAFFSVL RIYLSGWKGN PQLSDGLVYE
GFWEDPKEFA GGSAGQSSVF QCFDVLLGIQ QTAGGGHAAQ FLQDMRRYMP
PAHRNFLCSL ESNPSVREFV LSKGDAGLRE AYDACVKALV SLRSYHLQIV
TKYILIPASQ QPKENKTSED PSKLEAKGTG GTDLMNFLKT VRSTTEKSLL
KEG
UCSC uc064mfy.1; human.;

Category

Human IDO1 ELISA Kit

Human IDO-1 ELISA Kit;Human indoleamine-pyrrole 2,3-dioxygenase ELISA Kit;Human IDO ELISA Kit;Human INDO ELISA Kit;Human indoleamine 2,3-dioxygenase 1 ELISA Kit;Human indolamine 2,3 dioxygenase ELISA Kit;Human indole 2,3-dioxygenase ELISA Kit;