Human ITGB5 ELISA Kit

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  • Alternative name

    Human integrin subunit beta 5 ELISA Kit;Human integrin beta-5 ELISA Kit;Human testis secretory sperm-binding protein Li 217p ELISA Kit;

  • Catalog
    E020126
  • species
    Human
  • GeneITGB5
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Integrin Beta 5 (ITGb5). No significant cross-reactivity or interference between Integrin Beta 5 (ITGb5) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.055ng/mL.
  • Intended UseHuman ITGB5 ELISA Kit allows for the in vitro quantitative determination of ITGB5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Beta 5 (ITGb5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Beta 5 (ITGb5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Beta 5 (ITGb5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Integrin Beta 5 (ITGb5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Integrin beta-5 Protein information
  • Uniprot ID ITB5_HUMAN
  • Uniprot AC P18084; B0LPF8; B2RD70;
  • UniGene Hs.13155; Hs.536663;
  • GeneID 3693
  • KEGG hsa:3693;
  • Human Integrin beta-5 Protein SEQUENCE
  • SEQUENCE 799 AA; 88054 MW; D7E4727CA310512B CRC64;

    MPRAPAPLYA CLLGLCALLP RLAGLNICTS GSATSCEECL LIHPKCAWCS

    KEDFGSPRSI TSRCDLRANL VKNGCGGEIE SPASSFHVLR SLPLSSKGSG

    SAGWDVIQMT PQEIAVNLRP GDKTTFQLQV RQVEDYPVDL YYLMDLSLSM

    KDDLDNIRSL GTKLAEEMRK LTSNFRLGFG SFVDKDISPF SYTAPRYQTN

    PCIGYKLFPN CVPSFGFRHL LPLTDRVDSF NEEVRKQRVS RNRDAPEGGF

    DAVLQAAVCK EKIGWRKDAL HLLVFTTDDV PHIALDGKLG GLVQPHDGQC

    HLNEANEYTA SNQMDYPSLA LLGEKLAENN INLIFAVTKN HYMLYKNFTA

    LIPGTTVEIL DGDSKNIIQL IINAYNSIRS KVELSVWDQP EDLNLFFTAT

    CQDGVSYPGQ RKCEGLKIGD TASFEVSLEA RSCPSRHTEH VFALRPVGFR

    DSLEVGVTYN CTCGCSVGLE PNSARCNGSG TYVCGLCECS PGYLGTRCEC

    QDGENQSVYQ NLCREAEGKP LCSGRGDCSC NQCSCFESEF GKIYGPFCEC

    DNFSCARNKG VLCSGHGECH CGECKCHAGY IGDNCNCSTD ISTCRGRDGQ

    ICSERGHCLC GQCQCTEPGA FGEMCEKCPT CPDACSTKRD CVECLLLHSG

    KPDNQTCHSL CRDEVITWVD TIVKDDQEAV LCFYKTAKDC VMMFTYVELP

    SGKSNLTVLR EPECGNTPNA MTILLAVVGS ILLVGLALLA IWKLLVTIHD

    RREFAKFQSE RSRARYEMAS NPLYRKPIST HTVDFTFNKF NKSYNGTVD

  • UCSC uc003eho.4; human.;



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