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Alternative name
Interferon beta ELISA KIT; Fibroblast interferonIFNB1 ELISA KIT; IFB ELISA KIT; IFNB ELISA KIT; IFN-beta ELISA KIT
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Catalog
E020277
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species
Human
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GeneIFN-beta1/IFNB
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Human IFNB. No significant cross-reactivity or interference between Human IFNB and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe limit of detection of Human IFNB defined as the analyte concentration resulting in an absorbance significantly higher than that of the dilution medium (mean plus 2 standard deviations) was determined to be 15.6 pg/mL (mean of 6 independent assays).
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Intended UseHuman IFN-beta1/IFNB ELISA Kit allows for the in vitro quantitative determination of IFN-beta1/IFNB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: This assay employs a two-site sandwich ELISA to quantitative IFNB in Human serum, plasma. An antibody specific for IFNB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFNB present is bound by the immobilized antibody. After removing any unbound substances, a biotin - conjugated antibody specific for IFNB is added to the wells. After washing, Streptavidin conjugated Humanradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFNB bound in the initial step. The color development is stopped and the intensity of the color is measured.
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