Human HAVCR1 ELISA Kit

Alternative name
Human HAVCR-1 ELISA Kit;Human kidney injury molecule 1 ELISA Kit;Human KIM-1 ELISA Kit;Human T-cell immunoglobulin and mucin domain-containing protein 1 ELISA Kit;Human TIMD-1 ELISA Kit;Human T-cell immunoglobulin mucin receptor 1 ELISA Kit;Human TIM ELISA Kit;Human TIM-1 ELISA Kit;Human T-cell membrane protein 1 ELISA Kit;Human KIM1 ELISA Kit;Human TIM1 ELISA Kit;Human TIMD1 ELISA Kit;Human CD365 ELISA Kit;Human HAVCR ELISA Kit;Human hepatitis A virus cellular receptor 1 ELISA Kit;Human T cell immunoglobin domain and mucin domain protein 1 ELISA Kit;Human T-cell immunoglobulin mucin family member 1 ELISA Kit;
Catalog
E021794
species
Human
GeneHAVCR1
SpecificityThe Human KIM-1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human KIM-1 proteins within the range of 31.2 pg/ml - 2000 pg/ml.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity2 pg/ml.
Intended UseHuman HAVCR1 ELISA Kit allows for the in vitro quantitative determination of HAVCR1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||The Bioscience Human KIM-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human KIM-1 in Cell Culture Supernatants, Cell Lysates, Serum, Plasma, Urine. This assay employs an antibody specific for Human KIM-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and KIM-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human KIM-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of KIM-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: KIM1 (TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Mouses is encoded by the HAVCR1 gene. Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led Feigelstock et al. (1998) to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to mouse Tim1, Umetsu et al. (2005) showed that Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the mouse Tim1 gene and database analysis, McIntire et al. (2001) mapped the HAVCR1 gene to 5q33.2.
Human Hepatitis A virus cellular receptor 1 Protein information
Uniprot ID HAVR1_HUMAN
Uniprot AC Q96D42; O43656;
UniGene Hs.129711;
GeneID 26762
KEGG hsa:26762;
Human Hepatitis A virus cellular receptor 1 Protein SEQUENCE
SEQUENCE 359 AA; 38720 MW; D24BA2C932903ECA CRC64;
MHPQVVILSL ILHLADSVAG SVKVGGEAGP SVTLPCHYSG AVTSMCWNRG
SCSLFTCQNG IVWTNGTHVT YRKDTRYKLL GDLSRRDVSL TIENTAVSDS
GVYCCRVEHR GWFNDMKITV SLEIVPPKVT TTPIVTTVPT VTTVRTSTTV
PTTTTVPTTT VPTTMSIPTT TTVLTTMTVS TTTSVPTTTS IPTTTSVPVT
TTVSTFVPPM PLPRQNHEPV ATSPSSPQPA ETHPTTLQGA IRREPTSSPL
YSYTTDGNDT VTESSDGLWN NNQTQLFLEH SLLTANTTKG IYAGVCISVL
VLLALLGVII AKKYFFKKEV QQLSVSFSSL QIKALQNAVE KEVQAEDNIY
IENSLYATD
UCSC uc003lwi.3; human.;

Category

Human HAVCR1 ELISA Kit