Human PSTK ELISA Kit

Alternative name
Human O-phosphoseryl-tRNA(Sec) kinase ELISA Kit;Human C10orf89 ELISA Kit;Human phosphoseryl-tRNA kinase ELISA Kit;Human L-seryl-tRNA(Sec) kinase ELISA Kit;
Catalog
E022033
species
Human
GenePSTK
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.094ng/ml
Intended UseHuman PSTK ELISA Kit allows for the in vitro quantitative determination of PSTK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human PSTK antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PSTK antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PSTK amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human PSTK can be calculated.
Human L-seryl-tRNA(Sec) kinase Protein information
Uniprot ID PSTK_HUMAN
Uniprot AC Q8IV42; Q6ZSS9;
UniGene Hs.281004;
GeneID 118672
KEGG hsa:118672;
Human L-seryl-tRNA(Sec) kinase Protein SEQUENCE
SEQUENCE 348 AA; 39527 MW; A7DDFF0BDC93BE4E CRC64;
MKTAENIRGT GSDGPRKRGL CVLCGLPAAG KSTFARALAH RLQQEQGWAI
GVVAYDDVMP DAFLAGARAR PAPSQWKLLR QELLKYLEYF LMAVINGCQM
SVPPNRTEAM WEDFITCLKD QDLIFSAAFE AQSCYLLTKT AVSRPLFLVL
DDNFYYQSMR YEVYQLARKY SLGFCQLFLD CPLETCLQRN GQRPQALPPE
TIHLMGRKLE KPNPEKNAWE HNSLTIPSPA CASEASLEVT DLLLTALENP
VKYAEDNMEQ KDTDRIICST NILHKTDQTL RRIVSQTMKE AKGNQEAFSE
MTFKQRWVRA NHAAIWRIIL GNEHIKCRSA KVGWLQCCRI EKRPLSTG
UCSC uc001lgy.2; human. [Q8IV42-1];

Category

Human PSTK ELISA Kit

Human O-phosphoseryl-tRNA(Sec) kinase ELISA Kit;Human C10orf89 ELISA Kit;Human phosphoseryl-tRNA kinase ELISA Kit;Human L-seryl-tRNA(Sec) kinase ELISA Kit;