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Human LDHB ELISA Kit

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  • Alternative name

    Human LDH-B ELISA Kit;Human LDH heart subunit ELISA Kit;Human LDH-H ELISA Kit;Human renal carcinoma antigen NY-REN-46 ELISA Kit;Human HEL-S-281 ELISA Kit;Human LDHBD ELISA Kit;Human TRG-5 ELISA Kit;Human lactate dehydrogenase B ELISA Kit;Human L-lactate dehydrogenase B chain ELISA Kit;Human epididymis secretory protein Li 281 ELISA Kit;Human lactate dehydrogenase H chain ELISA Kit;Human testicular secretory protein Li 25 ELISA Kit;
  • Catalog
    E022075
  • species
    Human
  • GeneLDHB
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human LDHB. No significant cross-reactivity or interference between human LDHB and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human LDHB is typically less than 5.8 mU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
  • Intended UseHuman LDHB ELISA Kit allows for the in vitro quantitative determination of LDHB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Princple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LDHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LDHB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LDHB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LDHB bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human L-lactate dehydrogenase B chain Protein information
  • Uniprot ID LDHB_HUMAN
  • Uniprot AC P07195;
  • UniGene Hs.446149;
  • GeneID 3945
  • KEGG hsa:3945;
  • Human L-lactate dehydrogenase B chain Protein SEQUENCE

  • SEQUENCE 334 AA; 36638 MW; 3AD605DEED0D54A2 CRC64;

    MATLKEKLIA PVAEEEATVP NNKITVVGVG QVGMACAISI LGKSLADELA

    LVDVLEDKLK GEMMDLQHGS LFLQTPKIVA DKDYSVTANS KIVVVTAGVR

    QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCIIIVVSNP VDILTYVTWK

    LSGLPKHRVI GSGCNLDSAR FRYLMAEKLG IHPSSCHGWI LGEHGDSSVA

    VWSGVNVAGV SLQELNPEMG TDNDSENWKE VHKMVVESAY EVIKLKGYTN

    WAIGLSVADL IESMLKNLSR IHPVSTMVKG MYGIENEVFL SLPCILNARG

    LTSVINQKLK DDEVAQLKKS ADTLWDIQKD LKDL




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