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Alternative name
Proteasome subunit beta type-8 ELISA KIT; Low molecular mass protein 7 ELISA KIT; Macropain subunit C13 ELISA KIT; Multicatalytic endopeptidase complex subunit C13 ELISA KIT; Proteasome component C13 ELISA KIT; Proteasome subunit beta-5i ELISA KIT; Really interesting new gene 10 proteinPSMB8 ELISA KIT; LMP7 ELISA KIT; PSMB5i ELISA KIT; RING10 ELISA KIT; Y2 ELISA KIT
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Catalog
E022202
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species
Human
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GeneLMP7
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SpecificityThis assay has high sensitivity and excellent specificity for detection of LMP7. No significant cross-reactivity or interference between LMP7 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.117ng/mL.
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Intended UseHuman LMP7 ELISA Kit allows for the in vitro quantitative determination of LMP7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalIntended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of LMP7 in human tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to LMP7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to LMP7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LMP7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LMP7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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