Human LEFTY1 ELISA Kit

Alternative name
Human left-right determination factor B ELISA Kit;Human protein lefty-1 ELISA Kit;Human protein lefty-B ELISA Kit;Human LEFTB ELISA Kit;Human LEFTYB ELISA Kit;Human left-right determination factor 1 ELISA Kit;
Catalog
E022309
species
Human
GeneLEFTY1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Left/Right Determination Factor 1 (LEFTY1). No significant cross-reactivity or interference between Left/Right Determination Factor 1 (LEFTY1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.24ng/mL.
Intended UseHuman LEFTY1 ELISA Kit allows for the in vitro quantitative determination of LEFTY1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Left/Right Determination Factor 1 (LEFTY1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Left/Right Determination Factor 1 (LEFTY1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Left/Right Determination Factor 1 (LEFTY1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Left/Right Determination Factor 1 (LEFTY1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Left-right determination factor 1 Protein information
Uniprot ID LFTY1_HUMAN
Uniprot AC O75610; B2R7U0; Q53H67; Q5TE94;
UniGene Hs.656214;
GeneID 10637
KEGG hsa:10637;
Human Left-right determination factor 1 Protein SEQUENCE
SEQUENCE 366 AA; 40880 MW; BCF900C71ED9AA2A CRC64;
MQPLWLCWAL WVLPLASPGA ALTGEQLLGS LLRQLQLKEV PTLDRADMEE
LVIPTHVRAQ YVALLQRSHG DRSRGKRFSQ SFREVAGRFL ALEASTHLLV
FGMEQRLPPN SELVQAVLRL FQEPVPKAAL HRHGRLSPRS ARARVTVEWL
RVRDDGSNRT SLIDSRLVSV HESGWKAFDV TEAVNFWQQL SRPRQPLLLQ
VSVQREHLGP LASGAHKLVR FASQGAPAGL GEPQLELHTL DLGDYGAQGD
CDPEAPMTEG TRCCRQEMYI DLQGMKWAEN WVLEPPGFLA YECVGTCRQP
PEALAFKWPF LGPRQCIASE TDSLPMIVSI KEGGRTRPQV VSLPNMRVQK
CSCASDGALV PRRLQP
UCSC uc001hpo.3; human.;

Category

Human LEFTY1 ELISA Kit

Human left-right determination factor B ELISA Kit;Human protein lefty-1 ELISA Kit;Human protein lefty-B ELISA Kit;Human LEFTB ELISA Kit;Human LEFTYB ELISA Kit;Human left-right determination factor 1 ELISA Kit;