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Human LSP ELISA Kit

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  • Alternative name

    Lipoprotein signal peptidase ELISA KIT; Prolipoprotein signal peptidase ELISA KIT; Signal peptidase II ELISA KIT; SPase IIlspA ELISA KIT; lsp ELISA KIT; b0027 ELISA KIT; JW0025 ELISA KIT; SPase II ELISA KIT
  • Catalog
    E022909
  • species
    Human
  • GeneLSP
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman LSP ELISA Kit allows for the in vitro quantitative determination of LSP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyImmunology
  • Product Description
    specifical    Principle of the Assay: LPS ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for LPS. Standards or samples are then added to the microtiter plate wells and LPS if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of LPS present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for LPS are added to each well to "sandwich" the LPS immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain LPS and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LPS concentration in each sample is interpolated from this standard curve.



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