Human MPI ELISA Kit

Alternative name
Human phosphohexomutase ELISA Kit;Human Phosphomannose isomerase ELISA Kit;Human PMI ELISA Kit;Human PMI1 ELISA Kit;Human CDG1B ELISA Kit;Human mannose phosphate isomerase ELISA Kit;Human mannose-6-phosphate isomerase ELISA Kit;Human phosphomannose isomerase 1 ELISA Kit;
Catalog
E023607
species
Human
GeneMPI
SpecificityThis assay has high sensitivity and excellent specificity for detection of MPI. No significant cross-reactivity or interference between MPI and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of MPI is typically less than 6.4pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two st
Intended UseHuman MPI ELISA Kit allows for the in vitro quantitative determination of MPI , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MPI. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MPI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MPI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MPI in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Mannose-6-phosphate isomerase Protein information
Uniprot ID MPI_HUMAN
Uniprot AC P34949; A8K8K9; Q96AB0;
UniGene Hs.75694;
GeneID 4351
KEGG hsa:4351;
Human Mannose-6-phosphate isomerase Protein SEQUENCE
SEQUENCE 423 AA; 46656 MW; 1612DD966B86D3AC CRC64;
MAAPRVFPLS CAVQQYAWGK MGSNSEVARL LASSDPLAQI AEDKPYAELW
MGTHPRGDAK ILDNRISQKT LSQWIAENQD SLGSKVKDTF NGNLPFLFKV
LSVETPLSIQ AHPNKELAEK LHLQAPQHYP DANHKPEMAI ALTPFQGLCG
FRPVEEIVTF LKKVPEFQFL IGDEAATHLK QTMSHDSQAV ASSLQSCFSH
LMKSEKKVVV EQLNLLVKRI SQQAAAGNNM EDIFGELLLQ LHQQYPGDIG
CFAIYFLNLL TLKPGEAMFL EANVPHAYLK GDCVECMACS DNTVRAGLTP
KFIDVPTLCE MLSYTPSSSK DRLFLPTRSQ EDPYLSIYDP PVPDFTIMKT
EVPGSVTEYK VLALDSASIL LMVQGTVIAS TPTTQTPIPL QRGGVLFIGA
NESVSLKLTE PKDLLIFRAC CLL
UCSC uc002azc.3; human. [P34949-1];

Category

Human MPI ELISA Kit

Human phosphohexomutase ELISA Kit;Human Phosphomannose isomerase ELISA Kit;Human PMI ELISA Kit;Human PMI1 ELISA Kit;Human CDG1B ELISA Kit;Human mannose phosphate isomerase ELISA Kit;Human mannose-6-phosphate isomerase ELISA Kit;Human phosphomannose isomerase 1 ELISA Kit;