Human MGAT5 ELISA Kit

Alternative name
Human alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase ELISA Kit;Human glcNAc-T V ELISA Kit;Human GNT-V ELISA Kit;Human mannoside acetylglucosaminyltransferase 5 ELISA Kit;Human N-acetylglucosaminyl-transferase V ELISA Kit;Human GGNT5 ELISA Kit;Human GNT-VA ELISA Kit;Human mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase ELISA Kit;Human alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A ELISA Kit;
Catalog
E023635
species
Human
GeneMGAT5
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mannosyl Alpha-1,6-Glycoprotein Beta-1,6-N-Acetylglucosaminyltransferase (MGAT5). No significant cross-reactivity or interference between Mannosyl Alpha-1,6-Glycoprotein Beta-1,6-N-Acetylglucosaminyltransferase (MGAT5) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.067ng/mL.
Intended UseHuman MGAT5 ELISA Kit allows for the in vitro quantitative determination of MGAT5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannosyl Alpha-1,6-Glycoprotein Beta-1,6-N-Acetylglucosaminyltransferase (MGAT5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannosyl Alpha-1,6-Glycoprotein Beta-1,6-N-Acetylglucosaminyltransferase (MGAT5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannosyl Alpha-1,6-Glycoprotein Beta-1,6-N-Acetylglucosaminyltransferase (MGAT5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mannosyl Alpha-1,6-Glycoprotein Beta-1,6-N-Acetylglucosaminyltransferase (MGAT5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A Protein information
Uniprot ID MGT5A_HUMAN
Uniprot AC Q09328; D3DP70;
UniGene Hs.4988;
GeneID 4249
KEGG hsa:4249;
Human Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A Protein SEQUENCE
SEQUENCE 741 AA; 84543 MW; 1073F8E67AD0E23B CRC64;
MALFTPWKLS SQKLGFFLVT FGFIWGMMLL HFTIQQRTQP ESSSMLREQI
LDLSKRYIKA LAEENRNVVD GPYAGVMTAY DLKKTLAVLL DNILQRIGKL
ESKVDNLVVN GTGTNSTNST TAVPSLVALE KINVADIING AQEKCVLPPM
DGYPHCEGKI KWMKDMWRSD PCYADYGVDG STCSFFIYLS EVENWCPHLP
WRAKNPYEEA DHNSLAEIRT DFNILYSMMK KHEEFRWMRL RIRRMADAWI
QAIKSLAEKQ NLEKRKRKKV LVHLGLLTKE SGFKIAETAF SGGPLGELVQ
WSDLITSLYL LGHDIRISAS LAELKEIMKK VVGNRSGCPT VGDRIVELIY
IDIVGLAQFK KTLGPSWVHY QCMLRVLDSF GTEPEFNHAN YAQSKGHKTP
WGKWNLNPQQ FYTMFPHTPD NSFLGFVVEQ HLNSSDIHHI NEIKRQNQSL
VYGKVDSFWK NKKIYLDIIH TYMEVHATVY GSSTKNIPSY VKNHGILSGR
DLQFLLRETK LFVGLGFPYE GPAPLEAIAN GCAFLNPKFN PPKSSKNTDF
FIGKPTLREL TSQHPYAEVF IGRPHVWTVD LNNQEEVEDA VKAILNQKIE
PYMPYEFTCE GMLQRINAFI EKQDFCHGQV MWPPLSALQV KLAEPGQSCK
QVCQESQLIC EPSFFQHLNK DKDMLKYKVT CQSSELAKDI LVPSFDPKNK
HCVFQGDLLL FSCAGAHPRH QRVCPCRDFI KGQVALCKDC L
UCSC uc002ttw.5; human.;

Category

Human MGAT5 ELISA Kit