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Alternative name
Peroxiredoxin-5, mitochondrial ELISA KIT; Alu corepressor 1 ELISA KIT; Antioxidant enzyme B166 ELISA KIT; AOEB166 ELISA KIT; Liver tissue 2D-page spot 71B ELISA KIT; PLP ELISA KIT; Peroxiredoxin V ELISA KIT; Prx-V ELISA KIT; Peroxisomal antioxidant enzyme ELISA KIT; TPx type VI ELISA KIT; Thioredoxin peroxidase PMP20 ELISA KIT; Thioredoxin reductasePRDX5 ELISA KIT; ACR1 ELISA KIT; AOEB166 ELISA KIT; Prx-V ELISA KIT
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Catalog
E063285
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species
Mouse
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GenePrdx5
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity<0.469ng/ml
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Intended UseMouse Prdx5 ELISA Kit allows for the in vitro quantitative determination of Prdx5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Prdx5 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Prdx5 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Prdx5 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofMouse Prdx5 can be calculated.
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