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Human ACADM ELISA Kit

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  • Alternative name

    Human MCAD ELISA Kit;Human ACAD1 ELISA Kit;Human MCADH ELISA Kit;Human acyl-CoA dehydrogenase, C-4 to C-12 straight chain ELISA Kit;Human medium-chain specific acyl-CoA dehydrogenase, mitochondrial ELISA Kit;Human acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain ELISA Kit;Human testicular tissue protein Li 7 ELISA Kit;
  • Catalog
    E023948
  • species
    Human
  • GeneACADM
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ACADM. No significant cross-reactivity or interference between ACADM and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACADM and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman ACADM ELISA Kit allows for the in vitro quantitative determination of ACADM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Intended Uses: This ACADM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ACADM. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||ACADM ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-ACADM antibody and an ACADM-HRP conjugate. The assay sample and buffer are incubated together with ACADM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACADM concentration since ACADM from samples and ACADM-HRP conjugate compete for the anti-ACADM antibody binding site. Since the number of sites is limited, as more sites are occupied by ACADM from the sample, fewer sites are left to bind ACADM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACADM concentration in each sample is interpolated from this standard curve.
  • Human Medium-chain specific acyl-CoA dehydrogenase, mitochondrial Protein information
  • Uniprot ID ACADM_HUMAN
  • Uniprot AC P11310; Q5T4U4; Q9NYF1;
  • UniGene Hs.445040;
  • GeneID 34
  • KEGG hsa:34;
  • Human Medium-chain specific acyl-CoA dehydrogenase, mitochondrial Protein SEQUENCE

  • SEQUENCE 421 AA; 46588 MW; 7CD0B5832410581B CRC64;

    MAAGFGRCCR VLRSISRFHW RSQHTKANRQ REPGLGFSFE FTEQQKEFQA

    TARKFAREEI IPVAAEYDKT GEYPVPLIRR AWELGLMNTH IPENCGGLGL

    GTFDACLISE ELAYGCTGVQ TAIEGNSLGQ MPIIIAGNDQ QKKKYLGRMT

    EEPLMCAYCV TEPGAGSDVA GIKTKAEKKG DEYIINGQKM WITNGGKANW

    YFLLARSDPD PKAPANKAFT GFIVEADTPG IQIGRKELNM GQRCSDTRGI

    VFEDVKVPKE NVLIGDGAGF KVAMGAFDKT RPVVAAGAVG LAQRALDEAT

    KYALERKTFG KLLVEHQAIS FMLAEMAMKV ELARMSYQRA AWEVDSGRRN

    TYYASIAKAF AGDIANQLAT DAVQILGGNG FNTEYPVEKL MRDAKIYQIY

    EGTSQIQRLI VAREHIDKYK N

  • UCSC uc001dgw.6; human. [P11310-1];



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