Human MCM2 ELISA Kit

Alternative name
Human minichromosome maintenance protein 2 homolog ELISA Kit;Human nuclear protein BM28 ELISA Kit;Human BM28 ELISA Kit;Human CCNL1 ELISA Kit;Human CDCL1 ELISA Kit;Human KIAA0030 ELISA Kit;Human D3S3194 ELISA Kit;Human DFNA70 ELISA Kit;Human MITOTIN ELISA Kit;Human cdc19 ELISA Kit;Human minichromosome maintenance complex component 2 ELISA Kit;Human DNA replication licensing factor MCM2 ELISA Kit;Human cell devision cycle-like 1 ELISA Kit;Human cyclin-like 1 ELISA Kit;Human minichromosome maintenance deficient 2 (mitotin) ELISA Kit;
Catalog
E024580
species
Human
GeneMCM2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Minichromosome Maintenance Deficient 2 (MCM2). No significant cross-reactivity or interference between Minichromosome Maintenance Deficient 2 (MCM2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.055ng/mL.
Intended UseHuman MCM2 ELISA Kit allows for the in vitro quantitative determination of MCM2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Minichromosome Maintenance Deficient 2 (MCM2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Minichromosome Maintenance Deficient 2 (MCM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Minichromosome Maintenance Deficient 2 (MCM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Minichromosome Maintenance Deficient 2 (MCM2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human DNA replication licensing factor MCM2 Protein information
Uniprot ID MCM2_HUMAN
Uniprot AC P49736; Q14577; Q15023; Q8N2V1; Q969W7; Q96AE1; Q9BRM7;
UniGene Hs.477481;
GeneID 4171
KEGG hsa:4171;
Human DNA replication licensing factor MCM2 Protein SEQUENCE
SEQUENCE 904 AA; 101896 MW; 52C6DC61F128B404 CRC64;
MAESSESFTM ASSPAQRRRG NDPLTSSPGR SSRRTDALTS SPGRDLPPFE
DESEGLLGTE GPLEEEEDGE ELIGDGMERD YRAIPELDAY EAEGLALDDE
DVEELTASQR EAAERAMRQR DREAGRGLGR MRRGLLYDSD EEDEERPARK
RRQVERATED GEEDEEMIES IENLEDLKGH SVREWVSMAG PRLEIHHRFK
NFLRTHVDSH GHNVFKERIS DMCKENRESL VVNYEDLAAR EHVLAYFLPE
APAELLQIFD EAALEVVLAM YPKYDRITNH IHVRISHLPL VEELRSLRQL
HLNQLIRTSG VVTSCTGVLP QLSMVKYNCN KCNFVLGPFC QSQNQEVKPG
SCPECQSAGP FEVNMEETIY QNYQRIRIQE SPGKVAAGRL PRSKDAILLA
DLVDSCKPGD EIELTGIYHN NYDGSLNTAN GFPVFATVIL ANHVAKKDNK
VAVGELTDED VKMITSLSKD QQIGEKIFAS IAPSIYGHED IKRGLALALF
GGEPKNPGGK HKVRGDINVL LCGDPGTAKS QFLKYIEKVS SRAIFTTGQG
ASAVGLTAYV QRHPVSREWT LEAGALVLAD RGVCLIDEFD KMNDQDRTSI
HEAMEQQSIS ISKAGIVTSL QARCTVIAAA NPIGGRYDPS LTFSENVDLT
EPIISRFDIL CVVRDTVDPV QDEMLARFVV GSHVRHHPSN KEEEGLANGS
AAEPAMPNTY GVEPLPQEVL KKYIIYAKER VHPKLNQMDQ DKVAKMYSDL
RKESMATGSI PITVRHIESM IRMAEAHARI HLRDYVIEDD VNMAIRVMLE
SFIDTQKFSV MRSMRKTFAR YLSFRRDNNE LLLFILKQLV AEQVTYQRNR
FGAQQDTIEV PEKDLVDKAR QINIHNLSAF YDSELFRMNK FSHDLKRKMI
LQQF
UCSC uc003ejp.5; human.;

Category

Human MCM2 ELISA Kit