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Human MFN1 ELISA Kit

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  • Alternative name

    Human fzo homolog ELISA Kit;Human transmembrane GTPase MFN1 ELISA Kit;Human hfzo1 ELISA Kit;Human hfzo2 ELISA Kit;Human mitofusin 1 ELISA Kit;Human mitofusin-1 ELISA Kit;Human mitochondrial transmembrane GTPase FZO-2 ELISA Kit;Human mitochondrial transmembrane GTPase Fzo-1 ELISA Kit;Human putative transmembrane GTPase ELISA Kit;
  • Catalog
    E024692
  • species
    Human
  • GeneMFN1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mitofusin 1 (MFN1). No significant cross-reactivity or interference between Mitofusin 1 (MFN1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.057ng/mL.
  • Intended UseHuman MFN1 ELISA Kit allows for the in vitro quantitative determination of MFN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitofusin 1 (MFN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitofusin 1 (MFN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitofusin 1 (MFN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mitofusin 1 (MFN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Mitofusin-1 Protein information
  • Uniprot ID MFN1_HUMAN
  • Uniprot AC Q8IWA4; B2RAR1; D3DNR6; O15323; O60639; Q9BZB5; Q9NWQ2;
  • UniGene Hs.478383;
  • GeneID 55669
  • KEGG hsa:55669;
  • Human Mitofusin-1 Protein SEQUENCE

  • SEQUENCE 741 AA; 84100 MW; C4C1F56561D44A79 CRC64;

    MAEPVSPLKH FVLAKKAITA IFDQLLEFVT EGSHFVEATY KNPELDRIAT

    EDDLVEMQGY KDKLSIIGEV LSRRHMKVAF FGRTSSGKSS VINAMLWDKV

    LPSGIGHITN CFLSVEGTDG DKAYLMTEGS DEKKSVKTVN QLAHALHMDK

    DLKAGCLVRV FWPKAKCALL RDDLVLVDSP GTDVTTELDS WIDKFCLDAD

    VFVLVANSES TLMNTEKHFF HKVNERLSKP NIFILNNRWD ASASEPEYME

    DVRRQHMERC LHFLVEELKV VNALEAQNRI FFVSAKEVLS ARKQKAQGMP

    ESGVALAEGF HARLQEFQNF EQIFEECISQ SAVKTKFEQH TIRAKQILAT

    VKNIMDSVNL AAEDKRHYSV EEREDQIDRL DFIRNQMNLL TLDVKKKIKE

    VTEEVANKVS CAMTDEICRL SVLVDEFCSE FHPNPDVLKI YKSELNKHIE

    DGMGRNLADR CTDEVNALVL QTQQEIIENL KPLLPAGIQD KLHTLIPCKK

    FDLSYNLNYH KLCSDFQEDI VFPFSLGWSS LVHRFLGPRN AQRVLLGLSE

    PIFQLPRSLA STPTAPTTPA TPDNASQEEL MITLVTGLAS VTSRTSMGII

    IVGGVIWKTI GWKLLSVSLT MYGALYLYER LSWTTHAKER AFKQQFVNYA

    TEKLRMIVSS TSANCSHQVK QQIATTFARL CQQVDITQKQ LEEEIARLPK

    EIDQLEKIQN NSKLLRNKAV QLENELENFT KQFLPSSNEE S

  • UCSC uc003fjs.4; human. [Q8IWA4-1];



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