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Human MAPK13 ELISA Kit

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  • Alternative name

    Human MAP kinase 13 ELISA Kit;Human MAPK 13 ELISA Kit;Human mitogen-activated protein kinase p38 delta ELISA Kit;Human MAP kinase p38 delta ELISA Kit;Human stress-activated protein kinase 4 ELISA Kit;Human PRKM13 ELISA Kit;Human SAPK4 ELISA Kit;Human MAPK-13 ELISA Kit;Human p38delta ELISA Kit;Human mitogen-activated protein kinase 13 ELISA Kit;
  • Catalog
    E024701
  • species
    Human
  • GeneMAPK13
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mitogen Activated Protein Kinase 13 (MAPK13). No significant cross-reactivity or interference between Mitogen Activated Protein Kinase 13 (MAPK13) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.061ng/mL.
  • Intended UseHuman MAPK13 ELISA Kit allows for the in vitro quantitative determination of MAPK13 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitogen Activated Protein Kinase 13 (MAPK13). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitogen Activated Protein Kinase 13 (MAPK13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitogen Activated Protein Kinase 13 (MAPK13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mitogen Activated Protein Kinase 13 (MAPK13) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Mitogen-activated protein kinase 13 Protein information
  • Uniprot ID MK13_HUMAN
  • Uniprot AC O15264; O14739; O15124; Q5U4A5; Q6FI46; Q9UNU0;
  • UniGene Hs.178695;
  • GeneID 5603
  • KEGG hsa:5603;
  • Human Mitogen-activated protein kinase 13 Protein SEQUENCE

  • SEQUENCE 365 AA; 42090 MW; 52E749EDB2973DDF CRC64;

    MSLIRKKGFY KQDVNKTAWE LPKTYVSPTH VGSGAYGSVC SAIDKRSGEK

    VAIKKLSRPF QSEIFAKRAY RELLLLKHMQ HENVIGLLDV FTPASSLRNF

    YDFYLVMPFM QTDLQKIMGM EFSEEKIQYL VYQMLKGLKY IHSAGVVHRD

    LKPGNLAVNE DCELKILDFG LARHADAEMT GYVVTRWYRA PEVILSWMHY

    NQTVDIWSVG CIMAEMLTGK TLFKGKDYLD QLTQILKVTG VPGTEFVQKL

    NDKAAKSYIQ SLPQTPRKDF TQLFPRASPQ AADLLEKMLE LDVDKRLTAA

    QALTHPFFEP FRDPEEETEA QQPFDDSLEH EKLTVDEWKQ HIYKEIVNFS

    PIARKDSRRR SGMKL

  • UCSC uc003ols.5; human. [O15264-1];



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