Human MAP3K1 ELISA Kit

Alternative name
Human MAPK/ERK kinase kinase 1 ELISA Kit;Human MEK kinase 1 ELISA Kit;Human MEKK 1 ELISA Kit;Human MAPKKK1 ELISA Kit;Human MEKK ELISA Kit;Human MEKK1 ELISA Kit;Human SRXY6 ELISA Kit;Human mitogen-activated protein kinase kinase kinase 1 ELISA Kit;Human MAP/ERK kinase kinase 1 ELISA Kit;Human mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase ELISA Kit;
Catalog
E024742
species
Human
GeneMAP3K1
SpecificityThis assay has high sensitivity and excellent specificity for detection of human MAP3K1. No significant cross-reactivity or interference between human MAP3K1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human MAP3K1 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the m
Intended UseHuman MAP3K1 ELISA Kit allows for the in vitro quantitative determination of MAP3K1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MAP3K1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAP3K1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAP3K1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAP3K1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Mitogen-activated protein kinase kinase kinase 1 Protein information
Uniprot ID M3K1_HUMAN
Uniprot AC Q13233;
UniGene Hs.653654;
GeneID 4214
KEGG hsa:4214;
Human Mitogen-activated protein kinase kinase kinase 1 Protein SEQUENCE
SEQUENCE 1512 AA; 164470 MW; 5CB78242295411D9 CRC64;
MAAAAGNRAS SSGFPGARAT SPEAGGGGGA LKASSAPAAA AGLLREAGSG
GRERADWRRR QLRKVRSVEL DQLPEQPLFL AASPPASSTS PSPEPADAAG
SGTGFQPVAV PPPHGAASRG GAHLTESVAA PDSGASSPAA AEPGEKRAPA
AEPSPAAAPA GREMENKETL KGLHKMDDRP EERMIREKLK ATCMPAWKHE
WLERRNRRGP VVVKPIPVKG DGSEMNHLAA ESPGEVQASA ASPASKGRRS
PSPGNSPSGR TVKSESPGVR RKRVSPVPFQ SGRITPPRRA PSPDGFSPYS
PEETNRRVNK VMRARLYLLQ QIGPNSFLIG GDSPDNKYRV FIGPQNCSCA
RGTFCIHLLF VMLRVFQLEP SDPMLWRKTL KNFEVESLFQ KYHSRRSSRI
KAPSRNTIQK FVSRMSNSHT LSSSSTSTSS SENSIKDEEE QMCPICLLGM
LDEESLTVCE DGCRNKLHHH CMSIWAEECR RNREPLICPL CRSKWRSHDF
YSHELSSPVD SPSSLRAAQQ QTVQQQPLAG SRRNQESNFN LTHYGTQQIP
PAYKDLAEPW IQVFGMELVG CLFSRNWNVR EMALRRLSHD VSGALLLANG
ESTGNSGGSS GSSPSGGATS GSSQTSISGD VVEACCSVLS MVCADPVYKV
YVAALKTLRA MLVYTPCHSL AERIKLQRLL QPVVDTILVK CADANSRTSQ
LSISTLLELC KGQAGELAVG REILKAGSIG IGGVDYVLNC ILGNQTESNN
WQELLGRLCL IDRLLLEFPA EFYPHIVSTD VSQAEPVEIR YKKLLSLLTF
ALQSIDNSHS MVGKLSRRIY LSSARMVTTV PHVFSKLLEM LSVSSSTHFT
RMRRRLMAIA DEVEIAEAIQ LGVEDTLDGQ QDSFLQASVP NNYLETTENS
SPECTVHLEK TGKGLCATKL SASSEDISER LASISVGPSS STTTTTTTTE
QPKPMVQTKG RPHSQCLNSS PLSHHSQLMF PALSTPSSST PSVPAGTATD
VSKHRLQGFI PCRIPSASPQ TQRKFSLQFH RNCPENKDSD KLSPVFTQSR
PLPSSNIHRP KPSRPTPGNT SKQGDPSKNS MTLDLNSSSK CDDSFGCSSN
SSNAVIPSDE TVFTPVEEKC RLDVNTELNS SIEDLLEASM PSSDTTVTFK
SEVAVLSPEK AENDDTYKDD VNHNQKCKEK MEAEEEEALA IAMAMSASQD
ALPIVPQLQV ENGEDIIIIQ QDTPETLPGH TKAKQPYRED TEWLKGQQIG
LGAFSSCYQA QDVGTGTLMA VKQVTYVRNT SSEQEEVVEA LREEIRMMSH
LNHPNIIRML GATCEKSNYN LFIEWMAGGS VAHLLSKYGA FKESVVINYT
EQLLRGLSYL HENQIIHRDV KGANLLIDST GQRLRIADFG AAARLASKGT
GAGEFQGQLL GTIAFMAPEV LRGQQYGRSC DVWSVGCAII EMACAKPPWN
AEKHSNHLAL IFKIASATTA PSIPSHLSPG LRDVALRCLE LQPQDRPPSR
ELLKHPVFRT TW
UCSC uc003jqw.5; human.;

Category

Human MAP3K1 ELISA Kit