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Human ABCB4 ELISA Kit

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  • Alternative name

    Human ATP-binding cassette sub-family B member 4 ELISA Kit;Human multidrug resistance protein 3 ELISA Kit;Human P-glycoprotein 3 ELISA Kit;Human ABC21 ELISA Kit;Human GBD1 ELISA Kit;Human ICP3 ELISA Kit;Human MDR2 ELISA Kit;Human MDR2/3 ELISA Kit;Human MDR3 ELISA Kit;Human PFIC-3 ELISA Kit;Human PGY3 ELISA Kit;Human ATP binding cassette subfamily B member 4 ELISA Kit;Human phosphatidylcholine translocator ABCB4 ELISA Kit;Human ATP-binding cassette, sub-family B (MDR/TAP), member 4 ELISA Kit;Human P-glycoprotein-3/multiple drug resistance-3 ELISA Kit;Human multiple drug resistance 3 ELISA Kit;
  • Catalog
    E025122
  • species
    Human
  • GeneABCB4
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<46.9pg/ml
  • Intended UseHuman ABCB4 ELISA Kit allows for the in vitro quantitative determination of ABCB4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-human ABCB4antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-human ABCB4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ABCB4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofhuman ABCB4can be calculated.
  • Human Phosphatidylcholine translocator ABCB4 Protein information
  • Uniprot ID MDR3_HUMAN
  • Uniprot AC P21439; A0A2V7; A4D1D3; A4D1D4; A4D1D5; D6W5P3; D6W5P4; Q14813;
  • UniGene Hs.654403;
  • GeneID 5244
  • KEGG hsa:5244;
  • Human Phosphatidylcholine translocator ABCB4 Protein SEQUENCE

  • SEQUENCE 1286 AA; 141523 MW; 9A9066F2292F2CCF CRC64;

    MDLEAAKNGT AWRPTSAEGD FELGISSKQK RKKTKTVKMI GVLTLFRYSD

    WQDKLFMSLG TIMAIAHGSG LPLMMIVFGE MTDKFVDTAG NFSFPVNFSL

    SLLNPGKILE EEMTRYAYYY SGLGAGVLVA AYIQVSFWTL AAGRQIRKIR

    QKFFHAILRQ EIGWFDINDT TELNTRLTDD ISKISEGIGD KVGMFFQAVA

    TFFAGFIVGF IRGWKLTLVI MAISPILGLS AAVWAKILSA FSDKELAAYA

    KAGAVAEEAL GAIRTVIAFG GQNKELERYQ KHLENAKEIG IKKAISANIS

    MGIAFLLIYA SYALAFWYGS TLVISKEYTI GNAMTVFFSI LIGAFSVGQA

    APCIDAFANA RGAAYVIFDI IDNNPKIDSF SERGHKPDSI KGNLEFNDVH

    FSYPSRANVK ILKGLNLKVQ SGQTVALVGS SGCGKSTTVQ LIQRLYDPDE

    GTINIDGQDI RNFNVNYLRE IIGVVSQEPV LFSTTIAENI CYGRGNVTMD

    EIKKAVKEAN AYEFIMKLPQ KFDTLVGERG AQLSGGQKQR IAIARALVRN

    PKILLLDEAT SALDTESEAE VQAALDKARE GRTTIVIAHR LSTVRNADVI

    AGFEDGVIVE QGSHSELMKK EGVYFKLVNM QTSGSQIQSE EFELNDEKAA

    TRMAPNGWKS RLFRHSTQKN LKNSQMCQKS LDVETDGLEA NVPPVSFLKV

    LKLNKTEWPY FVVGTVCAIA NGGLQPAFSV IFSEIIAIFG PGDDAVKQQK

    CNIFSLIFLF LGIISFFTFF LQGFTFGKAG EILTRRLRSM AFKAMLRQDM

    SWFDDHKNST GALSTRLATD AAQVQGATGT RLALIAQNIA NLGTGIIISF

    IYGWQLTLLL LAVVPIIAVS GIVEMKLLAG NAKRDKKELE AAGKIATEAI

    ENIRTVVSLT QERKFESMYV EKLYGPYRNS VQKAHIYGIT FSISQAFMYF

    SYAGCFRFGA YLIVNGHMRF RDVILVFSAI VFGAVALGHA SSFAPDYAKA

    KLSAAHLFML FERQPLIDSY SEEGLKPDKF EGNITFNEVV FNYPTRANVP

    VLQGLSLEVK KGQTLALVGS SGCGKSTVVQ LLERFYDPLA GTVFVDFGFQ

    LLDGQEAKKL NVQWLRAQLG IVSQEPILFD CSIAENIAYG DNSRVVSQDE

    IVSAAKAANI HPFIETLPHK YETRVGDKGT QLSGGQKQRI AIARALIRQP

    QILLLDEATS ALDTESEKVV QEALDKAREG RTCIVIAHRL STIQNADLIV

    VFQNGRVKEH GTHQQLLAQK GIYFSMVSVQ AGTQNL

  • UCSC uc003uiv.2; human. [P21439-1];



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