Human NGLY1 ELISA Kit

Alternative name
Human PNGase ELISA Kit;Human hPNGase ELISA Kit;Human N-glycanase 1 ELISA Kit;Human peptide:N-glycanase ELISA Kit;Human PNG1 ELISA Kit;Human CDDG ELISA Kit;Human CDG1V ELISA Kit;Human peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase ELISA Kit;
Catalog
E025667
species
Human
GeneNGLY1
SpecificityThis assay has high sensitivity and excellent specificity for detection of human NGLY1. No significant cross-reactivity or interference between human NGLY1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human NGLY1 is typically less than 9.38 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
Intended UseHuman NGLY1 ELISA Kit allows for the in vitro quantitative determination of NGLY1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NGLY1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NGLY1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NGLY1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NGLY1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase Protein information
Uniprot ID NGLY1_HUMAN
Uniprot AC Q96IV0; B4DJE9; Q59FB1; Q6PJD8; Q9BVR8; Q9NR70;
UniGene Hs.368960;
GeneID 55768
KEGG hsa:55768;
Human Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase Protein SEQUENCE
SEQUENCE 654 AA; 74390 MW; 94BD44316EA66AF6 CRC64;
MAAAALGSSS GSASPAVAEL CQNTPETFLE ASKLLLTYAD NILRNPNDEK
YRSIRIGNTA FSTRLLPVRG AVECLFEMGF EEGETHLIFP KKASVEQLQK
IRDLIAIERS SRLDGSNKSH KVKSSQQPAA STQLPTTPSS NPSGLNQHTR
NRQGQSSDPP SASTVAADSA ILEVLQSNIQ HVLVYENPAL QEKALACIPV
QELKRKSQEK LSRARKLDKG INISDEDFLL LELLHWFKEE FFHWVNNVLC
SKCGGQTRSR DRSLLPSDDE LKWGAKEVED HYCDACQFSN RFPRYNNPEK
LLETRCGRCG EWANCFTLCC RAVGFEARYV WDYTDHVWTE VYSPSQQRWL
HCDACEDVCD KPLLYEIGWG KKLSYVIAFS KDEVVDVTWR YSCKHEEVIA
RRTKVKEALL RDTINGLNKQ RQLFLSENRR KELLQRIIVE LVEFISPKTP
KPGELGGRIS GSVAWRVARG EMGLQRKETL FIPCENEKIS KQLHLCYNIV
KDRYVRVSNN NQTISGWENG VWKMESIFRK VETDWHMVYL ARKEGSSFAY
ISWKFECGSV GLKVDSISIR TSSQTFQTGT VEWKLRSDTA QVELTGDNSL
HSYADFSGAT EVILEAELSR GDGDVAWQHT QLFRQSLNDH EENCLEIIIK
FSDL
UCSC uc003cdl.4; human. [Q96IV0-1];

Category

Human NGLY1 ELISA Kit

Human PNGase ELISA Kit;Human hPNGase ELISA Kit;Human N-glycanase 1 ELISA Kit;Human peptide:N-glycanase ELISA Kit;Human PNG1 ELISA Kit;Human CDDG ELISA Kit;Human CDG1V ELISA Kit;Human peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase ELISA Kit;