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Human NUMA1 ELISA Kit

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  • Alternative name

    Human NuMA protein ELISA Kit;Human nuclear matrix protein-22 ELISA Kit;Human NMP-22 ELISA Kit;Human SP-H antigen ELISA Kit;Human NUMA ELISA Kit;Human nuclear mitotic apparatus protein 1 ELISA Kit;Human centrophilin stabilizes mitotic spindle in mitotic cells ELISA Kit;Human structural nuclear protein ELISA Kit;
  • Catalog
    E026944
  • species
    Human
  • GeneNUMA1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Nuclear Mitotic Apparatus Protein 1 (NUMA1). No significant cross-reactivity or interference between Nuclear Mitotic Apparatus Protein 1 (NUMA1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.063ng/mL.
  • Intended UseHuman NUMA1 ELISA Kit allows for the in vitro quantitative determination of NUMA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Mitotic Apparatus Protein 1 (NUMA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Mitotic Apparatus Protein 1 (NUMA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Mitotic Apparatus Protein 1 (NUMA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nuclear Mitotic Apparatus Protein 1 (NUMA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Nuclear mitotic apparatus protein 1 Protein information
  • Uniprot ID NUMA1_HUMAN
  • Uniprot AC Q14980; H0YH75; Q14981; Q9BTE9;
  • UniGene Hs.325978; Hs.591967;
  • GeneID 4926
  • KEGG hsa:4926;
  • Human Nuclear mitotic apparatus protein 1 Protein SEQUENCE

  • SEQUENCE 2115 AA; 238260 MW; DE734EC85B812CC7 CRC64;

    MTLHATRGAA LLSWVNSLHV ADPVEAVLQL QDCSIFIKII DRIHGTEEGQ

    QILKQPVSER LDFVCSFLQK NRKHPSSPEC LVSAQKVLEG SELELAKMTM

    LLLYHSTMSS KSPRDWEQFE YKIQAELAVI LKFVLDHEDG LNLNEDLENF

    LQKAPVPSTC SSTFPEELSP PSHQAKREIR FLELQKVASS SSGNNFLSGS

    PASPMGDILQ TPQFQMRRLK KQLADERSNR DELELELAEN RKLLTEKDAQ

    IAMMQQRIDR LALLNEKQAA SPLEPKELEE LRDKNESLTM RLHETLKQCQ

    DLKTEKSQMD RKINQLSEEN GDLSFKLREF ASHLQQLQDA LNELTEEHSK

    ATQEWLEKQA QLEKELSAAL QDKKCLEEKN EILQGKLSQL EEHLSQLQDN

    PPQEKGEVLG DVLQLETLKQ EAATLAANNT QLQARVEMLE TERGQQEAKL

    LAERGHFEEE KQQLSSLITD LQSSISNLSQ AKEELEQASQ AHGARLTAQV

    ASLTSELTTL NATIQQQDQE LAGLKQQAKE KQAQLAQTLQ QQEQASQGLR

    HQVEQLSSSL KQKEQQLKEV AEKQEATRQD HAQQLATAAE EREASLRERD

    AALKQLEALE KEKAAKLEIL QQQLQVANEA RDSAQTSVTQ AQREKAELSR

    KVEELQACVE TARQEQHEAQ AQVAELELQL RSEQQKATEK ERVAQEKDQL

    QEQLQALKES LKVTKGSLEE EKRRAADALE EQQRCISELK AETRSLVEQH

    KRERKELEEE RAGRKGLEAR LQQLGEAHQA ETEVLRRELA EAMAAQHTAE

    SECEQLVKEV AAWRERYEDS QQEEAQYGAM FQEQLMTLKE ECEKARQELQ

    EAKEKVAGIE SHSELQISRQ QNELAELHAN LARALQQVQE KEVRAQKLAD

    DLSTLQEKMA ATSKEVARLE TLVRKAGEQQ ETASRELVKE PARAGDRQPE

    WLEEQQGRQF CSTQAALQAM EREAEQMGNE LERLRAALME SQGQQQEERG

    QQEREVARLT QERGRAQADL ALEKAARAEL EMRLQNALNE QRVEFATLQE

    ALAHALTEKE GKDQELAKLR GLEAAQIKEL EELRQTVKQL KEQLAKKEKE

    HASGSGAQSE AAGRTEPTGP KLEALRAEVS KLEQQCQKQQ EQADSLERSL

    EAERASRAER DSALETLQGQ LEEKAQELGH SQSALASAQR ELAAFRTKVQ

    DHSKAEDEWK AQVARGRQEA ERKNSLISSL EEEVSILNRQ VLEKEGESKE

    LKRLVMAESE KSQKLEERLR LLQAETASNS ARAAERSSAL REEVQSLREE

    AEKQRVASEN LRQELTSQAE RAEELGQELK AWQEKFFQKE QALSTLQLEH

    TSTQALVSEL LPAKHLCQQL QAEQAAAEKR HREELEQSKQ AAGGLRAELL

    RAQRELGELI PLRQKVAEQE RTAQQLRAEK ASYAEQLSML KKAHGLLAEE

    NRGLGERANL GRQFLEVELD QAREKYVQEL AAVRADAETR LAEVQREAQS

    TARELEVMTA KYEGAKVKVL EERQRFQEER QKLTAQVEQL EVFQREQTKQ

    VEELSKKLAD SDQASKVQQQ KLKAVQAQGG ESQQEAQRLQ AQLNELQAQL

    SQKEQAAEHY KLQMEKAKTH YDAKKQQNQE LQEQLRSLEQ LQKENKELRA

    EAERLGHELQ QAGLKTKEAE QTCRHLTAQV RSLEAQVAHA DQQLRDLGKF

    QVATDALKSR EPQAKPQLDL SIDSLDLSCE EGTPLSITSK LPRTQPDGTS

    VPGEPASPIS QRLPPKVESL ESLYFTPIPA RSQAPLESSL DSLGDVFLDS

    GRKTRSARRR TTQIINITMT KKLDVEEPDS ANSSFYSTRS APASQASLRA

    TSSTQSLARL GSPDYGNSAL LSLPGYRPTT RSSARRSQAG VSSGAPPGRN

    SFYMGTCQDE PEQLDDWNRI AELQQRNRVC PPHLKTCYPL ESRPSLSLGT

    ITDEEMKTGD PQETLRRASM QPIQIAEGTG ITTRQQRKRV SLEPHQGPGT

    PESKKATSCF PRPMTPRDRH EGRKQSTTEA QKKAAPASTK QADRRQSMAF

    SILNTPKKLG NSLLRRGASK KALSKASPNT RSGTRRSPRI ATTTASAATA

    AAIGATPRAK GKAKH

  • UCSC uc001ork.3; human. [Q14980-1];



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