Human NUSAP1 ELISA Kit

Alternative name
Human NUSAP ELISA Kit;Human ANKT ELISA Kit;Human BM037 ELISA Kit;Human LNP ELISA Kit;Human PRO0310p1 ELISA Kit;Human Q0310 ELISA Kit;Human SAPL ELISA Kit;Human nucleolar and spindle associated protein 1 ELISA Kit;Human nucleolar and spindle-associated protein 1 ELISA Kit;Human nucleolar protein ANKT ELISA Kit;
Catalog
E027043
species
Human
GeneNUSAP1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.094ng/ml
Intended UseHuman NUSAP1 ELISA Kit allows for the in vitro quantitative determination of NUSAP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-NUSAP1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-NUSAP1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the NUSAP1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of NUSAP1 can be calculated.
Human Nucleolar and spindle-associated protein 1 Protein information
Uniprot ID NUSAP_HUMAN
Uniprot AC Q9GZM9; Q9NZ85; Q9UI70;
UniGene Hs.615092;
GeneID 51203
KEGG hsa:51203;
Human Nucleolar and spindle-associated protein 1 Protein SEQUENCE
SEQUENCE 441 AA; 49452 MW; 1400E1B9F3FB727F CRC64;
MIIPSLEELD SLKYSDLQNL AKSLGLRANL RATKLLKALK GYIKHEARKG
NENQDESQTS ASSCDETEIQ ISNQEEAERQ PLGHVTKTRR RCKTVRVDPD
SQQNHSEIKI SNPTEFQNHE KQESQDLRAT AKVPSPPDEH QEAENAVSSG
NRDSKVPSEG KKSLYTDESS KPGKNKRTAI TTPNFKKLHE AHFKEMESID
QYIERKKKHF EEHNSMNELK QQPINKGGVR TPVPPRGRLS VASTPISQRR
SQGRSCGPAS QSTLGLKGSL KRSAISAAKT GVRFSAATKD NEHKRSLTKT
PARKSAHVTV SGGTPKGEAV LGTHKLKTIT GNSAAVITPF KLTTEATQTP
VSNKKPVFDL KASLSRPLNY EPHKGKLKPW GQSKENNYLN QHVNRINFYK
KTYKQPHLQT KEEQRKKREQ ERKEKKAKVL GMRRGLILAE D
UCSC uc001znr.5; human. [Q9BXS6-1];

Category

Human NUSAP1 ELISA Kit