Mouse Rag2 ELISA Kit

Alternative name
Mouse Rag-2 ELISA Kit;Mouse recombination activating gene 2 ELISA Kit;Mouse V(D)J recombination-activating protein 2 ELISA Kit;Mouse recombinase activating gene 2 ELISA Kit;Mouse recombination activating protein 2 ELISA Kit;
Catalog
E072217
species
Mouse
GeneRag2
SpecificityThis assay recognizes recombinant and natural mouse RAG-2. No significant cross-reactivity or interference was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Rag2 ELISA Kit allows for the in vitro quantitative determination of Rag2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAG-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for RAG-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain RAG-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of RAG-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse V(D)J recombination-activating protein 2 Protein information
Uniprot ID RAG2_MOUSE
Uniprot AC P21784;
UniGene Mm.4988;
GeneID 19374
KEGG mmu:19374;
Mouse V(D)J recombination-activating protein 2 Protein SEQUENCE
SEQUENCE 527 AA; 59074 MW; 51086F95A4A664A7 CRC64;
MSLQMVTVGH NIALIQPGFS LMNFDGQVFF FGQKGWPKRS CPTGVFHFDI
KQNHLKLKPA IFSKDSCYLP PLRYPATCSY KGSIDSDKHQ YIIHGGKTPN
NELSDKIYIM SVACKNNKKV TFRCTEKDLV GDVPEPRYGH SIDVVYSRGK
SMGVLFGGRS YMPSTQRTTE KWNSVADCLP HVFLIDFEFG CATSYILPEL
QDGLSFHVSI ARNDTVYILG GHSLASNIRP ANLYRIRVDL PLGTPAVNCT
VLPGGISVSS AILTQTNNDE FVIVGGYQLE NQKRMVCSLV SLGDNTIEIS
EMETPDWTSD IKHSKIWFGS NMGNGTIFLG IPGDNKQAMS EAFYFYTLRC
SEEDLSEDQK IVSNSQTSTE DPGDSTPFED SEEFCFSAEA TSFDGDDEFD
TYNEDDEDDE SVTGYWITCC PTCDVDINTW VPFYSTELNK PAMIYCSHGD
GHWVHAQCMD LEERTLIHLS EGSNKYYCNE HVQIARALQT PKRNPPLQKP
PMKSLHKKGS GKVLTPAKKS FLRRLFD
UCSC uc008lhj.1; mouse.;

Category

Mouse Rag2 ELISA Kit

Mouse Rag-2 ELISA Kit;Mouse recombination activating gene 2 ELISA Kit;Mouse V(D)J recombination-activating protein 2 ELISA Kit;Mouse recombinase activating gene 2 ELISA Kit;Mouse recombination activating protein 2 ELISA Kit;