Human OTC ELISA Kit

Alternative name
Human ornithine transcarbamylase ELISA Kit;Human OTCase ELISA Kit;Human OCTD ELISA Kit;Human ornithine carbamoyltransferase ELISA Kit;Human ornithine carbamoyltransferase, mitochondrial ELISA Kit;
Catalog
E027787
species
Human
GeneOTC
SpecificityThis assay has high sensitivity and excellent specificity for detection of human OTC. No significant cross-reactivity or interference between human OTC and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human OTC is typically less than 0.312 mU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
Intended UseHuman OTC ELISA Kit allows for the in vitro quantitative determination of OTC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrincple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for OTC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any OTC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for OTC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OTC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Ornithine carbamoyltransferase, mitochondrial Protein information
Uniprot ID OTC_HUMAN
Uniprot AC P00480; A8K9P2; D3DWB0; Q3KNR1; Q6B0I1; Q9NYJ5;
UniGene Hs.117050;
GeneID 5009
KEGG hsa:5009;
Human Ornithine carbamoyltransferase, mitochondrial Protein SEQUENCE
SEQUENCE 354 AA; 39935 MW; AE15B734F6E27A3B CRC64;
MLFNLRILLN NAAFRNGHNF MVRNFRCGQP LQNKVQLKGR DLLTLKNFTG
EEIKYMLWLS ADLKFRIKQK GEYLPLLQGK SLGMIFEKRS TRTRLSTETG
FALLGGHPCF LTTQDIHLGV NESLTDTARV LSSMADAVLA RVYKQSDLDT
LAKEASIPII NGLSDLYHPI QILADYLTLQ EHYSSLKGLT LSWIGDGNNI
LHSIMMSAAK FGMHLQAATP KGYEPDASVT KLAEQYAKEN GTKLLLTNDP
LEAAHGGNVL ITDTWISMGQ EEEKKKRLQA FQGYQVTMKT AKVAASDWTF
LHCLPRKPEE VDDEVFYSPR SLVFPEAENR KWTIMAVMVS LLTDYSPQLQ
KPKF
UCSC uc004def.5; human.;

Category

Human OTC ELISA Kit

Human ornithine transcarbamylase ELISA Kit;Human OTCase ELISA Kit;Human OCTD ELISA Kit;Human ornithine carbamoyltransferase ELISA Kit;Human ornithine carbamoyltransferase, mitochondrial ELISA Kit;