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Human RAP1A ELISA Kit

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  • Alternative name

    Human C21KG ELISA Kit;Human G-22K ELISA Kit;Human GTP-binding protein smg p21A ELISA Kit;Human Ras-related protein Krev-1 ELISA Kit;Human KREV1 ELISA Kit;Human KREV-1 ELISA Kit;Human RAP1 ELISA Kit;Human SMGP21 ELISA Kit;Human RAP1A, member of RAS oncogene family ELISA Kit;Human ras-related protein Rap-1A ELISA Kit;
  • Catalog
    E032930
  • species
    Human
  • GeneRAP1A
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.094ng/ml
  • Intended UseHuman RAP1A ELISA Kit allows for the in vitro quantitative determination of RAP1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human RAP1A antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human RAP1A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human RAP1A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human RAP1A can be calculated.
  • Human Ras-related protein Rap-1A Protein information
  • Uniprot ID RAP1A_HUMAN
  • Uniprot AC P62834; P10113;
  • UniGene Hs.190334; Hs.586618;
  • GeneID 5906
  • KEGG hsa:5906;
  • Human Ras-related protein Rap-1A Protein SEQUENCE

  • SEQUENCE 184 AA; 20987 MW; 42C39290C98E0A92 CRC64;

    MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDCQQ

    CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI

    LRVKDTEDVP MILVGNKCDL EDERVVGKEQ GQNLARQWCN CAFLESSAKS

    KINVNEIFYD LVRQINRKTP VEKKKPKKKS CLLL

  • UCSC uc001ebi.4; human.;



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