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Human PDCD1 ELISA Kit

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  • Alternative name

    Human protein PD-1 ELISA Kit;Human hPD-1 ELISA Kit;Human PD1 ELISA Kit;Human CD279 ELISA Kit;Human PD-1 ELISA Kit;Human SLEB2 ELISA Kit;Human hPD-l ELISA Kit;Human hSLE1 ELISA Kit;Human programmed cell death 1 ELISA Kit;Human programmed cell death protein 1 ELISA Kit;Human programmed cell death 1 protein ELISA Kit;Human systemic lupus erythematosus susceptibility 2 ELISA Kit;
  • Catalog
    E028409
  • species
    Human
  • GenePDCD1
  • SpecificityNatural and recombinant human PD-1
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 10pg/ml
  • Intended UseHuman PDCD1 ELISA Kit allows for the in vitro quantitative determination of PDCD1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: human PD-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PD-1 has been precoated onto 96-well plates. Standards(NSO, L25-Q167) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PD-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PD-1 amount of sample captured in plate. Background: Programmed cell death protein 1, also called PD-1 or PDCD1 is a protein that in humans is encoded by the PDCD1 gene. This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. By fluorescence in situ hybridization, the PDCD1 gene is mapped to 2q37.3. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.
  • Human Programmed cell death protein 1 Protein information
  • Uniprot ID PDCD1_HUMAN
  • Uniprot AC Q15116; O00517; Q8IX89;
  • UniGene Hs.158297;
  • GeneID 5133
  • KEGG hsa:5133;
  • Human Programmed cell death protein 1 Protein SEQUENCE

  • SEQUENCE 288 AA; 31647 MW; A5210FD40C304FB7 CRC64;

    MQIPQAPWPV VWAVLQLGWR PGWFLDSPDR PWNPPTFSPA LLVVTEGDNA

    TFTCSFSNTS ESFVLNWYRM SPSNQTDKLA AFPEDRSQPG QDCRFRVTQL

    PNGRDFHMSV VRARRNDSGT YLCGAISLAP KAQIKESLRA ELRVTERRAE

    VPTAHPSPSP RPAGQFQTLV VGVVGGLLGS LVLLVWVLAV ICSRAARGTI

    GARRTGQPLK EDPSAVPVFS VDYGELDFQW REKTPEPPVP CVPEQTEYAT

    IVFPSGMGTS SPARRGSADG PRSAQPLRPE DGHCSWPL

  • UCSC uc002wcq.5; human.;



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