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Human ASAH1 ELISA Kit

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  • Alternative name

    Human AC ELISA Kit;Human ACDase ELISA Kit;Human acid CDase ELISA Kit;Human acylsphingosine deacylase ELISA Kit;Human N-acylsphingosine amidohydrolase ELISA Kit;Human putative 32 kDa heart protein ELISA Kit;Human PHP32 ELISA Kit;Human ASAH ELISA Kit;Human PHP ELISA Kit;Human SMAPME ELISA Kit;Human N-acylsphingosine amidohydrolase 1 ELISA Kit;Human acid ceramidase ELISA Kit;Human N-acylsphingosine amidohydrolase (acid ceramidase) 1 ELISA Kit;
  • Catalog
    E000285
  • species
    Human
  • GeneASAH1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ASAH-1 No significant cross-reactivity or interference between ASAH-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ASAH-1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman ASAH1 ELISA Kit allows for the in vitro quantitative determination of ASAH1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: ASAH-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti- ASAH-1 antibody and an ASAH-1-HRP conjugate. The assay sample and buffer are incubated together with ASAH-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ASAH-1 concentration since ASAH-1 from samples and ASAH-1-HRP conjugate compete for the anti- ASAH-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ASAH-1 from the sample, fewer sites are left to bind ASAH-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ASAH-1 concentration in each sample is interpolated from this standard curve.
  • Human Acid ceramidase subunit beta Protein information
  • Uniprot ID ASAH1_HUMAN
  • Uniprot AC Q13510; E9PDS0; Q6W898; Q96AS2;
  • UniGene Hs.527412; Hs.633993;
  • GeneID 427
  • KEGG hsa:427;
  • Human Acid ceramidase subunit beta Protein SEQUENCE

  • SEQUENCE 395 AA; 44660 MW; 83467DBE8917DB6D CRC64;

    MPGRSCVALV LLAAAVSCAV AQHAPPWTED CRKSTYPPSG PTYRGAVPWY

    TINLDLPPYK RWHELMLDKA PVLKVIVNSL KNMINTFVPS GKIMQVVDEK

    LPGLLGNFPG PFEEEMKGIA AVTDIPLGEI ISFNIFYELF TICTSIVAED

    KKGHLIHGRN MDFGVFLGWN INNDTWVITE QLKPLTVNLD FQRNNKTVFK

    ASSFAGYVGM LTGFKPGLFS LTLNERFSIN GGYLGILEWI LGKKDVMWIG

    FLTRTVLENS TSYEEAKNLL TKTKILAPAY FILGGNQSGE GCVITRDRKE

    SLDVYELDAK QGRWYVVQTN YDRWKHPFFL DDRRTPAKMC LNRTSQENIS

    FETMYDVLST KPVLNKLTVY TTLIDVTKGQ FETYLRDCPD PCIGW

  • UCSC uc003wyl.3; human. [Q13510-1];



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