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Human PTK2 ELISA Kit

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  • Alternative name

    Human FADK 1 ELISA Kit;Human focal adhesion kinase-related nonkinase ELISA Kit;Human FRNK ELISA Kit;Human protein phosphatase 1 regulatory subunit 71 ELISA Kit;Human PPP1R71 ELISA Kit;Human Protein-tyrosine kinase 2 ELISA Kit;Human p125FAK ELISA Kit;Human pp125FAK ELISA Kit;Human FAK ELISA Kit;Human FAK1 ELISA Kit;Human FADK ELISA Kit;Human protein tyrosine kinase 2 ELISA Kit;Human focal adhesion kinase 1 ELISA Kit;Human FAK-related non-kinase polypeptide ELISA Kit;Human PTK2 protein tyrosine kinase 2 ELISA Kit;Human focal adhesion kinase isoform FAK-Del33 ELISA Kit;
  • Catalog
    E032270
  • species
    Human
  • GenePTK2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human PTK2. No significant cross-reactivity or interference between Human PTK2 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.039 ng/ml
  • Intended UseHuman PTK2 ELISA Kit allows for the in vitro quantitative determination of PTK2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Introduction: Focal adhesion kinase (FAK) is a protein tyrosine kinase which is recruited at an early stage to focal adhesions and which mediates many of the downstream responses. The kinase activity of FAK is activated by the Src non-receptor tyrosine kinase, which is recruited at an SH2 binding site generated on FAK by autophosphorylation. FAK subsequently interacts with a number of down-stream signalling proteins, including the adaptor protein Grb2 and the p85 _-subunit of phosphatidylinositol 3 kinase (PI3 kinase). Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FAK. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for FAK and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain FAK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of FAK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Focal adhesion kinase 1 Protein information
  • Uniprot ID FAK1_HUMAN
  • Uniprot AC Q05397; B4E2N6; F5H4S4; J3QT16; Q14291; Q8IYN9; Q9UD85;
  • UniGene Hs.395482;
  • GeneID 5747
  • KEGG hsa:5747;
  • Human Focal adhesion kinase 1 Protein SEQUENCE

  • SEQUENCE 1052 AA; 119233 MW; D8A4C15138AB0243 CRC64;

    MAAAYLDPNL NHTPNSSTKT HLGTGMERSP GAMERVLKVF HYFESNSEPT

    TWASIIRHGD ATDVRGIIQK IVDSHKVKHV ACYGFRLSHL RSEEVHWLHV

    DMGVSSVREK YELAHPPEEW KYELRIRYLP KGFLNQFTED KPTLNFFYQQ

    VKSDYMLEIA DQVDQEIALK LGCLEIRRSY WEMRGNALEK KSNYEVLEKD

    VGLKRFFPKS LLDSVKAKTL RKLIQQTFRQ FANLNREESI LKFFEILSPV

    YRFDKECFKC ALGSSWIISV ELAIGPEEGI SYLTDKGCNP THLADFTQVQ

    TIQYSNSEDK DRKGMLQLKI AGAPEPLTVT APSLTIAENM ADLIDGYCRL

    VNGTSQSFII RPQKEGERAL PSIPKLANSE KQGMRTHAVS VSETDDYAEI

    IDEEDTYTMP STRDYEIQRE RIELGRCIGE GQFGDVHQGI YMSPENPALA

    VAIKTCKNCT SDSVREKFLQ EALTMRQFDH PHIVKLIGVI TENPVWIIME

    LCTLGELRSF LQVRKYSLDL ASLILYAYQL STALAYLESK RFVHRDIAAR

    NVLVSSNDCV KLGDFGLSRY MEDSTYYKAS KGKLPIKWMA PESINFRRFT

    SASDVWMFGV CMWEILMHGV KPFQGVKNND VIGRIENGER LPMPPNCPPT

    LYSLMTKCWA YDPSRRPRFT ELKAQLSTIL EEEKAQQEER MRMESRRQAT

    VSWDSGGSDE APPKPSRPGY PSPRSSEGFY PSPQHMVQTN HYQVSGYPGS

    HGITAMAGSI YPGQASLLDQ TDSWNHRPQE IAMWQPNVED STVLDLRGIG

    QVLPTHLMEE RLIRQQQEME EDQRWLEKEE RFLKPDVRLS RGSIDREDGS

    LQGPIGNQHI YQPVGKPDPA APPKKPPRPG APGHLGSLAS LSSPADSYNE

    GVKLQPQEIS PPPTANLDRS NDKVYENVTG LVKAVIEMSS KIQPAPPEEY

    VPMVKEVGLA LRTLLATVDE TIPLLPASTH REIEMAQKLL NSDLGELINK

    MKLAQQYVMT SLQQEYKKQM LTAAHALAVD AKNLLDVIDQ ARLKMLGQTR

    PH

  • UCSC uc003yvu.4; human. [Q05397-1];



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