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Human PGAM2 ELISA Kit

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  • Alternative name

    Human BPG-dependent PGAM 2 ELISA Kit;Human muscle-specific phosphoglycerate mutase ELISA Kit;Human phosphoglycerate mutase isozyme M ELISA Kit;Human PGAM-M ELISA Kit;Human PGAMM ELISA Kit;Human GSD10 ELISA Kit;Human phosphoglycerate mutase 2 ELISA Kit;Human phosphoglycerate mutase 2 (muscle) ELISA Kit;
  • Catalog
    E029103
  • species
    Human
  • GenePGAM2
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman PGAM2 ELISA Kit allows for the in vitro quantitative determination of PGAM2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: PGAM2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PGAM2. Standards or samples are then added to the microtiter plate wells and PGAM2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PGAM2 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for PGAM2 are added to each well to "sandwich" the PGAM2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PGAM2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGAM2 concentration in each sample is interpolated from this standard curve.
  • Human Phosphoglycerate mutase 2 Protein information
  • Uniprot ID PGAM2_HUMAN
  • Uniprot AC P15259;
  • UniGene Hs.632642;
  • GeneID 5224
  • KEGG hsa:5224;
  • Human Phosphoglycerate mutase 2 Protein SEQUENCE

  • SEQUENCE 253 AA; 28766 MW; 0FDC97631104FCF7 CRC64;

    MATHRLVMVR HGESTWNQEN RFCGWFDAEL SEKGTEEAKR GAKAIKDAKM

    EFDICYTSVL KRAIRTLWAI LDGTDQMWLP VVRTWRLNER HYGGLTGLNK

    AETAAKHGEE QVKIWRRSFD IPPPPMDEKH PYYNSISKER RYAGLKPGEL

    PTCESLKDTI ARALPFWNEE IVPQIKAGKR VLIAAHGNSL RGIVKHLEGM

    SDQAIMELNL PTGIPIVYEL NKELKPTKPM QFLGDEETVR KAMEAVAAQG

    KAK

  • UCSC uc003tjs.3; human.;



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