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Human PIK3CB ELISA Kit

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  • Alternative name

    Human PI3-kinase subunit beta ELISA Kit;Human PI3K-beta ELISA Kit;Human PI3KBETA ELISA Kit;Human ptdIns-3-kinase subunit beta ELISA Kit;Human phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta ELISA Kit;Human ptdIns-3-kinase subunit p110-beta ELISA Kit;Human P110BETA ELISA Kit;Human PIK3C1 ELISA Kit;Human PI3K ELISA Kit;Human phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta ELISA Kit;Human phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform ELISA Kit;Human PI3-kinase p110 subunit beta ELISA Kit;Human PtdIns-3-kinase p110 ELISA Kit;Human phosphoinositide-3-kinase, catalytic, beta polypeptide ELISA Kit;
  • Catalog
    E029118
  • species
    Human
  • GenePIK3CB
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb). No significant cross-reactivity or interference between Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.059ng/mL.
  • Intended UseHuman PIK3CB ELISA Kit allows for the in vitro quantitative determination of PIK3CB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform Protein information
  • Uniprot ID PK3CB_HUMAN
  • Uniprot AC P42338; D3DNF0; Q24JU2;
  • UniGene Hs.239818;
  • GeneID 5291
  • KEGG hsa:5291;
  • Human Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform Protein SEQUENCE

  • SEQUENCE 1070 AA; 122762 MW; 81135FE93452C00E CRC64;

    MCFSFIMPPA MADILDIWAV DSQIASDGSI PVDFLLPTGI YIQLEVPREA

    TISYIKQMLW KQVHNYPMFN LLMDIDSYMF ACVNQTAVYE ELEDETRRLC

    DVRPFLPVLK LVTRSCDPGE KLDSKIGVLI GKGLHEFDSL KDPEVNEFRR

    KMRKFSEEKI LSLVGLSWMD WLKQTYPPEH EPSIPENLED KLYGGKLIVA

    VHFENCQDVF SFQVSPNMNP IKVNELAIQK RLTIHGKEDE VSPYDYVLQV

    SGRVEYVFGD HPLIQFQYIR NCVMNRALPH FILVECCKIK KMYEQEMIAI

    EAAINRNSSN LPLPLPPKKT RIISHVWENN NPFQIVLVKG NKLNTEETVK

    VHVRAGLFHG TELLCKTIVS SEVSGKNDHI WNEPLEFDIN ICDLPRMARL

    CFAVYAVLDK VKTKKSTKTI NPSKYQTIRK AGKVHYPVAW VNTMVFDFKG

    QLRTGDIILH SWSSFPDELE EMLNPMGTVQ TNPYTENATA LHVKFPENKK

    QPYYYPPFDK IIEKAAEIAS SDSANVSSRG GKKFLPVLKE ILDRDPLSQL

    CENEMDLIWT LRQDCREIFP QSLPKLLLSI KWNKLEDVAQ LQALLQIWPK

    LPPREALELL DFNYPDQYVR EYAVGCLRQM SDEELSQYLL QLVQVLKYEP

    FLDCALSRFL LERALGNRRI GQFLFWHLRS EVHIPAVSVQ FGVILEAYCR

    GSVGHMKVLS KQVEALNKLK TLNSLIKLNA VKLNRAKGKE AMHTCLKQSA

    YREALSDLQS PLNPCVILSE LYVEKCKYMD SKMKPLWLVY NNKVFGEDSV

    GVIFKNGDDL RQDMLTLQML RLMDLLWKEA GLDLRMLPYG CLATGDRSGL

    IEVVSTSETI ADIQLNSSNV AAAAAFNKDA LLNWLKEYNS GDDLDRAIEE

    FTLSCAGYCV ASYVLGIGDR HSDNIMVKKT GQLFHIDFGH ILGNFKSKFG

    IKRERVPFIL TYDFIHVIQQ GKTGNTEKFG RFRQCCEDAY LILRRHGNLF

    ITLFALMLTA GLPELTSVKD IQYLKDSLAL GKSEEEALKQ FKQKFDEALR

    ESWTTKVNWM AHTVRKDYRS

  • UCSC uc011bmq.4; human.;



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