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Mouse Aurkaip1 ELISA Kit

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  • Alternative name

    Mouse AURKA-interacting protein ELISA Kit;Mouse 28S ribosomal protein S38, mitochondrial ELISA Kit;Mouse MRP-S38 ELISA Kit;Mouse Aip ELISA Kit;Mouse Akip ELISA Kit;Mouse Mrps38 ELISA Kit;Mouse 0610033H09Rik ELISA Kit;Mouse aurora kinase A interacting protein 1 ELISA Kit;Mouse aurora kinase A-interacting protein ELISA Kit;Mouse Aurora-A kinase interacting protein ELISA Kit;
  • Catalog
    E045291
  • species
    Mouse
  • GeneAurkaip1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of AURKAIP1. No significant cross-reactivity or interference between AURKAIP1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AURKAIP1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Aurkaip1 ELISA Kit allows for the in vitro quantitative determination of Aurkaip1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical    Principle of the assay: AURKAIP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AURKAIP1 antibody and an AURKAIP1-HRP conjugate. The assay sample and buffer are incubated together with AURKAIP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AURKAIP1 concentration since AURKAIP1 from samples and AURKAIP1-HRP conjugate compete for the anti-AURKAIP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by AURKAIP1 from the sample, fewer sites are left to bind AURKAIP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AURKAIP1 concentration in each sample is interpolated from this standard curve.
  • Mouse Aurora kinase A-interacting protein Protein information
  • Uniprot ID AKIP_MOUSE
  • Uniprot AC Q9DCJ7; Q9D1J7;
  • UniGene Mm.28999;
  • GeneID 66077
  • KEGG mmu:66077;
  • Mouse Aurora kinase A-interacting protein Protein SEQUENCE

  • SEQUENCE 200 AA; 23300 MW; FA26D360169CC160 CRC64;

    MFLARLTSRL ARTVVPWAGF SRSCPGSGVI GSYAFRPLYS LQPASPSRAA

    SLPGKRTQSE LEEFLVPRKM AISPLESWLT AQYLLPRRNV EVPVTLAPSQ

    FYECPPRQGE EEAQQGVREA WDATPVQCKN VLKIRRRKMN HHKYRKLVKR

    TRFLRRKVRE GRLKKKQIKF EKDLKRIWLK AGLKEAPENW QTPKIYLKNK

  • UCSC uc008wey.2; mouse.;



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