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Human PLCZ1 ELISA Kit

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  • Alternative name

    Human phosphoinositide phospholipase C-zeta-1 ELISA Kit;Human Phospholipase C-zeta-1 ELISA Kit;Human PLC-zeta-1 ELISA Kit;Human Testis-development protein NYD-SP27 ELISA Kit;Human NYD-SP27 ELISA Kit;Human PLCzeta ELISA Kit;Human SPGF17 ELISA Kit;Human phospholipase C zeta 1 ELISA Kit;Human 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 ELISA Kit;Human PI-phospholipase C zeta 1 ELISA Kit;Human testicular tissue protein Li 145 ELISA Kit;Human testis-development related NYD-SP27 ELISA Kit;
  • Catalog
    E029214
  • species
    Human
  • GenePLCZ1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Phospholipase C Zeta 1 (PLCz1). No significant cross-reactivity or interference between Phospholipase C Zeta 1 (PLCz1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.117ng/mL.
  • Intended UseHuman PLCZ1 ELISA Kit allows for the in vitro quantitative determination of PLCZ1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase C Zeta 1 (PLCz1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase C Zeta 1 (PLCz1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase C Zeta 1 (PLCz1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase C Zeta 1 (PLCz1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 Protein information
  • Uniprot ID PLCZ1_HUMAN
  • Uniprot AC Q86YW0; Q08AQ7; Q96J70;
  • UniGene Hs.743832; Hs.97542;
  • GeneID 89869
  • KEGG hsa:89869;
  • Human 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1 Protein SEQUENCE

  • SEQUENCE 608 AA; 70411 MW; 540A81A2DDB9537B CRC64;

    MEMRWFLSKI QDDFRGGKIN LEKTQRLLEK LDIRCSYIHV KQIFKDNDRL

    KQGRITIEEF RAIYRIITHR EEIIEIFNTY SENRKILLAS NLAQFLTQEQ

    YAAEMSKAIA FEIIQKYEPI EEVRKAHQMS LEGFTRYMDS RECLLFKNEC

    RKVYQDMTHP LNDYFISSSH NTYLVSDQLL GPSDLWGYVS ALVKGCRCLE

    IDCWDGAQNE PVVYHGYTLT SKLLFKTVIQ AIHKYAFMTS DYPVVLSLEN

    HCSTAQQEVM ADNLQATFGE SLLSDMLDDF PDTLPSPEAL KFKILVKNKK

    IGTLKETHER KGSDKRGDNQ DKETGVKKLP GVMLFKKKKT RKLKIALALS

    DLVIYTKAEK FKSFQHSRLY QQFNENNSIG ETQARKLSKL RVHEFIFHTR

    KFITRIYPKA TRADSSNFNP QEFWNIGCQM VALNFQTPGL PMDLQNGKFL

    DNGGSGYILK PHFLRESKSY FNPSNIKEGM PITLTIRLIS GIQLPLTHSS

    SNKGDSLVII EVFGVPNDQM KQQTRVIKKN AFSPRWNETF TFIIHVPELA

    LIRFVVEGQG LIAGNEFLGQ YTLPLLCMNK GYRRIPLFSR MGESLEPASL

    FVYVWYVR

  • UCSC uc058lrt.1; human. [Q86YW0-1];



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