Human PLOD3 ELISA Kit

Alternative name
Human lysyl hydroxylase 3 ELISA Kit;Human LH3 ELISA Kit;Human procollagen-lysine,2-oxoglutarate 5-dioxygenase 3 ELISA Kit;Human lysine hydroxylase 3 ELISA Kit;Human lysyl hydroxlase 3 ELISA Kit;
Catalog
E030921
species
Human
GenePLOD3
SpecificityThis assay has high sensitivity and excellent specificity for detection of PLOD3. No significant cross-reactivity or interference between PLOD3 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human PLOD3 is typically less than 0.106ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by add
Intended UseHuman PLOD3 ELISA Kit allows for the in vitro quantitative determination of PLOD3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PLOD3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to PLOD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PLOD3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PLOD3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Procollagen-lysine,2-oxoglutarate 5-dioxygenase 3 Protein information
Uniprot ID PLOD3_HUMAN
Uniprot AC O60568; B2R6W6; Q540C3;
UniGene Hs.153357;
GeneID 8985
KEGG hsa:8985;
Human Procollagen-lysine,2-oxoglutarate 5-dioxygenase 3 Protein SEQUENCE
SEQUENCE 738 AA; 84785 MW; 08424B46985941F9 CRC64;
MTSSGPGPRF LLLLPLLLPP AASASDRPRG RDPVNPEKLL VITVATAETE
GYLRFLRSAE FFNYTVRTLG LGEEWRGGDV ARTVGGGQKV RWLKKEMEKY
ADREDMIIMF VDSYDVILAG SPTELLKKFV QSGSRLLFSA ESFCWPEWGL
AEQYPEVGTG KRFLNSGGFI GFATTIHQIV RQWKYKDDDD DQLFYTRLYL
DPGLREKLSL NLDHKSRIFQ NLNGALDEVV LKFDRNRVRI RNVAYDTLPI
VVHGNGPTKL QLNYLGNYVP NGWTPEGGCG FCNQDRRTLP GGQPPPRVFL
AVFVEQPTPF LPRFLQRLLL LDYPPDRVTL FLHNNEVFHE PHIADSWPQL
QDHFSAVKLV GPEEALSPGE ARDMAMDLCR QDPECEFYFS LDADAVLTNL
QTLRILIEEN RKVIAPMLSR HGKLWSNFWG ALSPDEYYAR SEDYVELVQR
KRVGVWNVPY ISQAYVIRGD TLRMELPQRD VFSGSDTDPD MAFCKSFRDK
GIFLHLSNQH EFGRLLATSR YDTEHLHPDL WQIFDNPVDW KEQYIHENYS
RALEGEGIVE QPCPDVYWFP LLSEQMCDEL VAEMEHYGQW SGGRHEDSRL
AGGYENVPTV DIHMKQVGYE DQWLQLLRTY VGPMTESLFP GYHTKARAVM
NFVVRYRPDE QPSLRPHHDS STFTLNVALN HKGLDYEGGG CRFLRYDCVI
SSPRKGWALL HPGRLTHYHE GLPTTWGTRY IMVSFVDP
UCSC uc003uyd.4; human.;

Category

Human PLOD3 ELISA Kit

Human lysyl hydroxylase 3 ELISA Kit;Human LH3 ELISA Kit;Human procollagen-lysine,2-oxoglutarate 5-dioxygenase 3 ELISA Kit;Human lysine hydroxylase 3 ELISA Kit;Human lysyl hydroxlase 3 ELISA Kit;