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Alternative name
Prokineticin receptor 1 ELISA KIT; G-protein coupled receptor 73 ELISA KIT; G-protein coupled receptor ZAQ ELISA KIT; GPR73aPROKR1 ELISA KIT; GPR73 ELISA KIT; PKR1 ELISA KIT; PK-R1 ELISA KIT
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Catalog
E031061
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species
Human
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GenePKR1
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Prokineticin Receptor 1 (PKR1).
No significant cross-reactivity or interference between Prokineticin Receptor 1 (PKR1) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.228ng/mL.
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Intended UseHuman PKR1 ELISA Kit allows for the in vitro quantitative determination of PKR1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prokineticin Receptor 1 (PKR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prokineticin Receptor 1 (PKR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prokineticin Receptor 1 (PKR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prokineticin Receptor 1 (PKR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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