Human PRKAA1 ELISA Kit

Alternative name
Human AMPK subunit alpha-1 ELISA Kit;Human acetyl-CoA carboxylase kinase ELISA Kit;Human ACACA kinase ELISA Kit;Human hydroxymethylglutaryl-CoA reductase kinase ELISA Kit;Human HMGCR kinase ELISA Kit;Human tau-protein kinase PRKAA1 ELISA Kit;Human AMPK1 ELISA Kit;Human AMPK ELISA Kit;Human AMPKa1 ELISA Kit;Human protein kinase AMP-activated catalytic subunit alpha 1 ELISA Kit;Human 5'-AMP-activated protein kinase catalytic subunit alpha-1 ELISA Kit;Human 5'-AMP-activated protein kinase, catalytic alpha-1 chain ELISA Kit;Human AMP -activate kinase alpha 1 subunit ELISA Kit;Human AMP-activated protein kinase, catalytic, alpha-1 ELISA Kit;Human AMPK alpha 1 ELISA Kit;Human protein kinase, AMP-activated, alpha 1 catalytic subunit ELISA Kit;
Catalog
E031651
species
Human
GenePRKAA1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Protein Kinase, AMP Activated Alpha 1 (PRKAa1). No significant cross-reactivity or interference between Protein Kinase, AMP Activated Alpha 1 (PRKAa1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.067ng/mL.
Intended UseHuman PRKAA1 ELISA Kit allows for the in vitro quantitative determination of PRKAA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase, AMP Activated Alpha 1 (PRKAa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase, AMP Activated Alpha 1 (PRKAa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase, AMP Activated Alpha 1 (PRKAa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase, AMP Activated Alpha 1 (PRKAa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human 5'-AMP-activated protein kinase catalytic subunit alpha-1 Protein information
Uniprot ID AAPK1_HUMAN
Uniprot AC Q13131; A8MTQ6; B2R7E1; O00286; Q5D0E1; Q86VS1; Q9UNQ4;
UniGene Hs.43322;
GeneID 5562
KEGG hsa:5562;
Human 5'-AMP-activated protein kinase catalytic subunit alpha-1 Protein SEQUENCE
SEQUENCE 559 AA; 64009 MW; ABAE71FBF912947A CRC64;
MRRLSSWRKM ATAEKQKHDG RVKIGHYILG DTLGVGTFGK VKVGKHELTG
HKVAVKILNR QKIRSLDVVG KIRREIQNLK LFRHPHIIKL YQVISTPSDI
FMVMEYVSGG ELFDYICKNG RLDEKESRRL FQQILSGVDY CHRHMVVHRD
LKPENVLLDA HMNAKIADFG LSNMMSDGEF LRTSCGSPNY AAPEVISGRL
YAGPEVDIWS SGVILYALLC GTLPFDDDHV PTLFKKICDG IFYTPQYLNP
SVISLLKHML QVDPMKRATI KDIREHEWFK QDLPKYLFPE DPSYSSTMID
DEALKEVCEK FECSEEEVLS CLYNRNHQDP LAVAYHLIID NRRIMNEAKD
FYLATSPPDS FLDDHHLTRP HPERVPFLVA ETPRARHTLD ELNPQKSKHQ
GVRKAKWHLG IRSQSRPNDI MAEVCRAIKQ LDYEWKVVNP YYLRVRRKNP
VTSTYSKMSL QLYQVDSRTY LLDFRSIDDE ITEAKSGTAT PQRSGSVSNY
RSCQRSDSDA EAQGKSSEVS LTSSVTSLDS SPVDLTPRPG SHTIEFFEMC
ANLIKILAQ
UCSC uc003jmb.4; human. [Q13131-1];

Category

Human PRKAA1 ELISA Kit