Human PRKG1 ELISA Kit

Alternative name
Human cGK 1 ELISA Kit;Human cGK1 ELISA Kit;Human cGMP-dependent protein kinase I ELISA Kit;Human cGKI ELISA Kit;Human PRKG1B ELISA Kit;Human PRKGR1A ELISA Kit;Human PRKGR1B ELISA Kit;Human AAT8 ELISA Kit;Human PKG ELISA Kit;Human cGK ELISA Kit;Human cGKI-BETA ELISA Kit;Human cGKI-alpha ELISA Kit;Human protein kinase, cGMP-dependent, type I ELISA Kit;Human cGMP-dependent protein kinase 1 ELISA Kit;Human protein kinase, cGMP-dependent, regulatory, type I, beta ELISA Kit;
Catalog
E031659
species
Human
GenePRKG1
SpecificityThis assay has high sensitivity and excellent specificity for detection of human PRKG1. No significant cross-reactivity or interference between human PRKG1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human PRKG1 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
Intended UseHuman PRKG1 ELISA Kit allows for the in vitro quantitative determination of PRKG1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PRKG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRKG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKG1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKG1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human cGMP-dependent protein kinase 1 Protein information
Uniprot ID KGP1_HUMAN
Uniprot AC Q13976; A5YM56; B3KSF3; E2PU10; P14619; Q5JP05; Q5JSJ6; Q6P5T7;
UniGene Hs.407535;
GeneID 5592
KEGG hsa:5592;
Human cGMP-dependent protein kinase 1 Protein SEQUENCE
SEQUENCE 671 AA; 76364 MW; 51389502A5E5FBD2 CRC64;
MSELEEDFAK ILMLKEERIK ELEKRLSEKE EEIQELKRKL HKCQSVLPVP
STHIGPRTTR AQGISAEPQT YRSFHDLRQA FRKFTKSERS KDLIKEAILD
NDFMKNLELS QIQEIVDCMY PVEYGKDSCI IKEGDVGSLV YVMEDGKVEV
TKEGVKLCTM GPGKVFGELA ILYNCTRTAT VKTLVNVKLW AIDRQCFQTI
MMRTGLIKHT EYMEFLKSVP TFQSLPEEIL SKLADVLEET HYENGEYIIR
QGARGDTFFI ISKGTVNVTR EDSPSEDPVF LRTLGKGDWF GEKALQGEDV
RTANVIAAEA VTCLVIDRDS FKHLIGGLDD VSNKAYEDAE AKAKYEAEAA
FFANLKLSDF NIIDTLGVGG FGRVELVQLK SEESKTFAMK ILKKRHIVDT
RQQEHIRSEK QIMQGAHSDF IVRLYRTFKD SKYLYMLMEA CLGGELWTIL
RDRGSFEDST TRFYTACVVE AFAYLHSKGI IYRDLKPENL ILDHRGYAKL
VDFGFAKKIG FGKKTWTFCG TPEYVAPEII LNKGHDISAD YWSLGILMYE
LLTGSPPFSG PDPMKTYNII LRGIDMIEFP KKIAKNAANL IKKLCRDNPS
ERLGNLKNGV KDIQKHKWFE GFNWEGLRKG TLTPPIIPSV ASPTDTSNFD
SFPEDNDEPP PDDNSGWDID F
UCSC uc001jjo.4; human. [Q13976-1];

Category

Human PRKG1 ELISA Kit