Human WNT10B ELISA Kit

Alternative name
Human Protein Wnt-12 ELISA Kit;Human WNT12 ELISA Kit;Human SHFM6 ELISA Kit;Human STHAG8 ELISA Kit;Human WNT-12 ELISA Kit;Human Wnt family member 10B ELISA Kit;Human protein Wnt-10b ELISA Kit;Human WNT-10B protein ELISA Kit;Human wingless-type MMTV integration site family, member 10B ELISA Kit;
Catalog
E031915
species
Human
GeneWNT10B
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.188ng/ml
Intended UseHuman WNT10B ELISA Kit allows for the in vitro quantitative determination of WNT10B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human WNT10B antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human WNT10B antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human WNT10B amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human WNT10B can be calculated.
Human Protein Wnt-10b Protein information
Uniprot ID WN10B_HUMAN
Uniprot AC O00744; B2R7A5; O00747; Q4VAJ4; Q4VAJ5; Q8WZ97;
UniGene Hs.91985;
GeneID 7480
KEGG hsa:7480;
Human Protein Wnt-10b Protein SEQUENCE
SEQUENCE 389 AA; 43000 MW; F973F2CA0DB115EF CRC64;
MLEEPRPRPP PSGLAGLLFL ALCSRALSNE ILGLKLPGEP PLTANTVCLT
LSGLSKRQLG LCLRNPDVTA SALQGLHIAV HECQHQLRDQ RWNCSALEGG
GRLPHHSAIL KRGFRESAFS FSMLAAGVMH AVATACSLGK LVSCGCGWKG
SGEQDRLRAK LLQLQALSRG KSFPHSLPSP GPGSSPSPGP QDTWEWGGCN
HDMDFGEKFS RDFLDSREAP RDIQARMRIH NNRVGRQVVT ENLKRKCKCH
GTSGSCQFKT CWRAAPEFRA VGAALRERLG RAIFIDTHNR NSGAFQPRLR
PRRLSGELVY FEKSPDFCER DPTMGSPGTR GRACNKTSRL LDGCGSLCCG
RGHNVLRQTR VERCHCRFHW CCYVLCDECK VTEWVNVCK
UCSC uc001rss.4; human. [O00744-1];

Category

Human WNT10B ELISA Kit

Human Protein Wnt-12 ELISA Kit;Human WNT12 ELISA Kit;Human SHFM6 ELISA Kit;Human STHAG8 ELISA Kit;Human WNT-12 ELISA Kit;Human Wnt family member 10B ELISA Kit;Human protein Wnt-10b ELISA Kit;Human WNT-10B protein ELISA Kit;Human wingless-type MMTV integration site family, member 10B ELISA Kit;