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Human WNT3A ELISA Kit

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  • Alternative name

    Human Wnt family member 3A ELISA Kit;Human protein Wnt-3a ELISA Kit;Human wingless-type MMTV integration site family, member 3A ELISA Kit;
  • Catalog
    E031922
  • species
    Human
  • GeneWNT3A
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human WNT3A. No significant cross-reactivity or interference between Human WNT3A and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.078 ng/mL (mean of 6 independent assays).
  • Intended UseHuman WNT3A ELISA Kit allows for the in vitro quantitative determination of WNT3A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Intended Uses: For the quantitative detection of Human Protein Wnt-3a (WNT3A) concentration in serum, plasma and other biological fluids. Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitative WNT3A in Human serum, plasma. An antibody specific for WNT3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin - conjugated antibody specific for WNT3A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT3A bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Protein Wnt-3a Protein information
  • Uniprot ID WNT3A_HUMAN
  • Uniprot AC P56704; Q3SY79; Q3SY80; Q969P2;
  • UniGene Hs.336930;
  • GeneID 89780
  • KEGG hsa:89780;
  • Human Protein Wnt-3a Protein SEQUENCE

  • SEQUENCE 352 AA; 39365 MW; A317BD6D4A73920B CRC64;

    MAPLGYFLLL CSLKQALGSY PIWWSLAVGP QYSSLGSQPI LCASIPGLVP

    KQLRFCRNYV EIMPSVAEGI KIGIQECQHQ FRGRRWNCTT VHDSLAIFGP

    VLDKATRESA FVHAIASAGV AFAVTRSCAE GTAAICGCSS RHQGSPGKGW

    KWGGCSEDIE FGGMVSREFA DARENRPDAR SAMNRHNNEA GRQAIASHMH

    LKCKCHGLSG SCEVKTCWWS QPDFRAIGDF LKDKYDSASE MVVEKHRESR

    GWVETLRPRY TYFKVPTERD LVYYEASPNF CEPNPETGSF GTRDRTCNVS

    SHGIDGCDLL CCGRGHNARA ERRREKCRCV FHWCCYVSCQ ECTRVYDVHT

    CK

  • UCSC uc001hrq.3; human. [P56704-1];



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