Human WNT5A ELISA Kit

Alternative name
Human hWNT5A ELISA Kit;Human Wnt family member 5A ELISA Kit;Human protein Wnt-5a ELISA Kit;Human WNT-5A protein ELISA Kit;Human wingless-type MMTV integration site family, member 5A ELISA Kit;
Catalog
E031926
species
Human
GeneWNT5A
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.094ng/ml
Intended UseHuman WNT5A ELISA Kit allows for the in vitro quantitative determination of WNT5A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human Protein Wnt-5a antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human Protein Wnt-5a antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Protein Wnt-5a amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human Protein Wnt-5a can be calculated.
Human Protein Wnt-5a Protein information
Uniprot ID WNT5A_HUMAN
Uniprot AC P41221; A8K4A4; Q6P278;
UniGene Hs.643085;
GeneID 7474
KEGG hsa:7474;
Human Protein Wnt-5a Protein SEQUENCE
SEQUENCE 380 AA; 42339 MW; 50E73AC7FE96C7B5 CRC64;
MKKSIGILSP GVALGMAGSA MSSKFFLVAL AIFFSFAQVV IEANSWWSLG
MNNPVQMSEV YIIGAQPLCS QLAGLSQGQK KLCHLYQDHM QYIGEGAKTG
IKECQYQFRH RRWNCSTVDN TSVFGRVMQI GSRETAFTYA VSAAGVVNAM
SRACREGELS TCGCSRAARP KDLPRDWLWG GCGDNIDYGY RFAKEFVDAR
ERERIHAKGS YESARILMNL HNNEAGRRTV YNLADVACKC HGVSGSCSLK
TCWLQLADFR KVGDALKEKY DSAAAMRLNS RGKLVQVNSR FNSPTTQDLV
YIDPSPDYCV RNESTGSLGT QGRLCNKTSE GMDGCELMCC GRGYDQFKTV
QTERCHCKFH WCCYVKCKKC TEIVDQFVCK
UCSC uc003dhn.5; human. [P41221-1];

Category

Human WNT5A ELISA Kit

Human hWNT5A ELISA Kit;Human Wnt family member 5A ELISA Kit;Human protein Wnt-5a ELISA Kit;Human WNT-5A protein ELISA Kit;Human wingless-type MMTV integration site family, member 5A ELISA Kit;